He Gui-zhen, Dong Liang-guang, Cui Xiao-yu, Chen Xue-feng, Shu Hong
Department of Enteral and Parenteral Nutrition, PUMC Hospital, CAMS and PUMC, Beijing, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2008 Sep;11(5):469-71.
To estimate the effect of the lymph duct ligation on systemic inflammatory factors and endotoxins during intestinal ischemia-reperfusion (I/R).
Male SD rats underwent occlusion of superior mesenteric artery for 60 min followed by reperfusion for 120 min plus lymph duct ligation or not. Forty rats were randomly divided into 4 groups: group A (blank); group B (sham); group C (intestinal I/R); group D (intestinal I/R plus lymph duct ligation). Mesenteric lymph nodes were harvested for standard bacteriologic cultures. The endotoxin, D-lactate, diamine oxidase (DAO), and cytokines in serum were detected.
The rates of bacterial translocation to mesenteric lymph nodes were 40% in group C and 20% in group D. No positive lymph node cultures were encountered in any of group A and B. The serum cytokines (except for sICAM-1) , D-lactate, DAO and endotoxin levels were lower in group D than those in group C (P<0.05), but both were higher than those in group A and B (P<0.05).
During intestinal I/R injury, blockage the lymph flow from gut into bloodstream decreases the levels of cytokines, and significantly attenuates the increase in intestinal permeability.
评估淋巴管结扎对肠缺血再灌注（I/R）期间全身炎症因子和内毒素的影响。
雄性SD大鼠行肠系膜上动脉阻断60分钟，随后再灌注120分钟，部分大鼠进行淋巴管结扎。40只大鼠随机分为4组：A组（空白组）；B组（假手术组）；C组（肠I/R组）；D组（肠I/R加淋巴管结扎组）。采集肠系膜淋巴结进行标准细菌培养。检测血清中的内毒素、D-乳酸、二胺氧化酶（DAO）和细胞因子。
C组肠系膜淋巴结细菌移位率为40%，D组为20%。A组和B组未发现淋巴结培养阳性。D组血清细胞因子（除sICAM-1外）、D-乳酸、DAO和内毒素水平低于C组（P<0.05），但均高于A组和B组（P<0.05）。
在肠I/R损伤期间，阻断肠道淋巴液进入血液循环可降低细胞因子水平，并显著减轻肠道通透性增加。
