Liu Chang, Wu Qifei, Li Quanyuan, Liu Dong, Su Haibo, Shen Naiying, Tai Minghui, Lv Yi
Department of Hepatobiliary Surgery, First Affiliated Hospital, School of Medicine, Xi'an Jiao Tong University, Xi'an, China.
J Surg Res. 2009 Jun 1;154(1):45-50. doi: 10.1016/j.jss.2008.06.006. Epub 2008 Jul 11.
Whether the mesenteric lymphatic system could serve as a route of transport by which gut-derived inflammatory mediators contribute to the induction of remote organ injuries is uncertain. We therefore made a gut-induced lung injury canine model by portal vein occlusion and reperfusion (PV O/R) and studied the role of mesenteric lymphatic ducts ligation (ML) to gut-induced lung injury with this model.
Eighteen mongrel dogs were divided into control, PV O/R, and PV O/R + ML groups. Cytokines and endotoxin levels in the portal vein and lymph from thoracic duct in different groups were tested. The permeability, myeloperoxidase activity, and histopathological investigation of intestine and lung were evaluated.
Cytokines and endotoxin levels in the portal vein were significantly increased in experimental groups compared with control group (P < 0.05), and that in the lymph from thoracic duct were significantly increased in PV O/R group compared with control and PV O/R + ML group (P < 0.05). Lung permeability and MPO activity in PV O/R group were significantly higher than those in control and PV O/R + ML group (P < 0.05); intestinal permeability in experimental groups were significantly higher with respect to control group. The lung injury score in PV O/R group was significantly higher than those in control and PV O/R + ML group (P < 0.05) and the intestinal injury scores in experimental groups were significantly higher than control group (P < 0.05).
The gut-induced lung injury canine model made by PV O/R is successful, and mesenteric lymphatic ducts ligation decreases the degree of gut-induced lung injury in this model.
肠系膜淋巴系统是否可作为肠道源性炎症介质导致远隔器官损伤的运输途径尚不确定。因此，我们通过门静脉阻断和再灌注（PV O/R）建立了犬肠道诱导性肺损伤模型，并利用该模型研究肠系膜淋巴管结扎（ML）对肠道诱导性肺损伤的作用。
18只杂种犬分为对照组、PV O/R组和PV O/R + ML组。检测不同组门静脉和胸导管淋巴中的细胞因子和内毒素水平。评估肠道和肺的通透性、髓过氧化物酶活性及组织病理学情况。
与对照组相比，实验组门静脉中的细胞因子和内毒素水平显著升高（P < 0.05）；与对照组和PV O/R + ML组相比，PV O/R组胸导管淋巴中的细胞因子和内毒素水平显著升高（P < 0.05）。PV O/R组的肺通透性和MPO活性显著高于对照组和PV O/R + ML组（P < 0.05）；实验组的肠道通透性相对于对照组显著升高。PV O/R组的肺损伤评分显著高于对照组和PV O/R + ML组（P < 0.05），实验组的肠道损伤评分显著高于对照组（P < 0.05）。
通过PV O/R建立的犬肠道诱导性肺损伤模型是成功的，在该模型中肠系膜淋巴管结扎可减轻肠道诱导性肺损伤的程度。
