Zhang Nan, Van Zele Thibaut, Perez-Novo Claudina, Van Bruaene Nicholas, Holtappels Gabriele, DeRuyck Natalie, Van Cauwenberge Paul, Bachert Claus
Upper Airway Research Laboratory (URL), Department of Oto-Rhino-Laryngology, Ghent University Hospital, Ghent, Belgium.
J Allergy Clin Immunol. 2008 Nov;122(5):961-8. doi: 10.1016/j.jaci.2008.07.008. Epub 2008 Sep 19.
Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by the accumulation of inflammatory cells; however, an eosinophil predominance is seen in white (Belgian), but not Asian (south Chinese), patients with polyps.
We sought to investigate the association of inflammatory cell predominance with regulatory T-cell and T-effector cell patterns.
Nasal mucosal tissue was obtained from 26 consecutive Belgian patients with CRSwNP and 21 Belgian control subjects and 29 south Chinese patients with CRSwNP and 29 south Chinese control subjects, who all underwent phenotyping, including nasal endoscopy and computed tomographic scanning. Tissues were investigated for granulocytes and their products and T-effector/regulatory T cells and related cytokines.
Both CRSwNP groups were comparable in terms of symptoms, computed tomographic scan results, and nasal endoscopy results, but asthma comorbidity was significantly higher in white patients. Tissue from white patients with CRSwNP was characterized by eosinophilic inflammation (eosinophil cationic protein/myeloperoxidase ratio > 2), whereas samples from Asian patients were biased toward neutrophilic inflammation (eosinophil cationic protein/myeloperoxidase ratio = 0.25). Both CRSwNP groups demonstrated significant upregulation of the T-cell activation marker soluble IL-2 receptor alpha and significant downregulation of Foxp3 expression and TGF-beta1 protein content versus their respective control groups. However, whereas white patients displayed a significant increase in T(H)2 cytokine and related marker levels versus control subjects and versus Asian patients, the latter showed a T(H)1/T(H)17 cell pattern versus control tissue.
Nasal polyps (CRSwNP) from white and Asian patients are both characterized by T-cell activation and impaired regulatory T-cell function; however, T-effector cells in the samples from white patients were T(H)2-biased, whereas samples from their Asian counterparts demonstrated a T(H)1/T(H)17 polarization.
伴鼻息肉的慢性鼻-鼻窦炎（CRSwNP）的特征为炎症细胞积聚；然而，在白种人（比利时人）鼻息肉患者中以嗜酸性粒细胞为主，而在亚洲人（中国南方人）鼻息肉患者中并非如此。
我们试图研究炎症细胞优势与调节性T细胞和效应性T细胞模式之间的关联。
从26例连续的比利时CRSwNP患者、21例比利时对照受试者、29例中国南方CRSwNP患者和29例中国南方对照受试者获取鼻黏膜组织，所有受试者均接受了包括鼻内镜检查和计算机断层扫描在内的表型分析。对组织进行粒细胞及其产物、效应性T细胞/调节性T细胞及相关细胞因子的研究。
两个CRSwNP组在症状、计算机断层扫描结果和鼻内镜检查结果方面具有可比性，但白种患者中哮喘合并症显著更高。白种CRSwNP患者的组织以嗜酸性粒细胞炎症为特征（嗜酸性粒细胞阳离子蛋白/髓过氧化物酶比值>2），而亚洲患者的样本则倾向于中性粒细胞炎症（嗜酸性粒细胞阳离子蛋白/髓过氧化物酶比值=0.25）。与各自的对照组相比，两个CRSwNP组均显示T细胞活化标志物可溶性IL-2受体α显著上调，Foxp3表达和TGF-β1蛋白含量显著下调。然而，与对照受试者和亚洲患者相比，白种患者的辅助性T细胞2（TH2）细胞因子及相关标志物水平显著升高，而后者与对照组织相比显示出辅助性T细胞1（TH1）/辅助性T细胞17（TH17）细胞模式。
白种人和亚洲患者的鼻息肉（CRSwNP）均以T细胞活化和调节性T细胞功能受损为特征；然而，白种患者样本中的效应性T细胞以TH2为主，而亚洲患者样本则表现为TH1/TH17极化。
