Shyu Y John, Hu Chang-Deng
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue Cancer Center, Purdue University, West Lafayette, IN 47907, USA.
Trends Biotechnol. 2008 Nov;26(11):622-30. doi: 10.1016/j.tibtech.2008.07.006. Epub 2008 Sep 17.
Numerous technologies based on utilizing fluorescent proteins have been developed for biological research, and fluorescence complementation (FC) is a recent application for visualization of molecular events in living cells and organisms. Currently, ten fluorescent proteins have been demonstrated to support FC. Over the past five years, FC-based technologies have been developed to visualize a variety of molecular events, such as protein-protein interactions, post-translational modifications, protein folding, conformational changes, RNA-protein interactions, mRNA localization and DNA hybridization. In addition, FC has also been used for drug discovery. These applications are providing fascinating insights into many biological processes. Here, we review the principles and applications of FC technologies, discuss their current challenges and examine prospects for future advances.
基于利用荧光蛋白的众多技术已被开发用于生物学研究，荧光互补（FC）是用于可视化活细胞和生物体中分子事件的最新应用。目前，已证明有十种荧光蛋白支持荧光互补。在过去五年中，基于荧光互补的技术已被开发用于可视化各种分子事件，如蛋白质-蛋白质相互作用、翻译后修饰、蛋白质折叠、构象变化、RNA-蛋白质相互作用、mRNA定位和DNA杂交。此外，荧光互补还被用于药物发现。这些应用为许多生物学过程提供了引人入胜的见解。在这里，我们回顾荧光互补技术的原理和应用，讨论其当前面临的挑战并审视未来进展的前景。
