Mannina L, Sobolev A P, Capitani D, Iaffaldano N, Rosato M P, Ragni P, Reale A, Sorrentino E, D'Amico I, Coppola R
Dipartimento STAAM, Università degli Studi del Molise, Via De Sanctis, 86100 Campobasso, Italy.
Talanta. 2008 Oct 19;77(1):433-44. doi: 10.1016/j.talanta.2008.07.006. Epub 2008 Jul 15.
The nuclear magnetic resonance (NMR) technique was used as analytical tool to determine the complete metabolic profiling of sea bass extracts: water-soluble metabolites belonging to different classes such as sugars, amino acids, dipeptides and organic acids as well as metabolites soluble in organic solvent such as lipids, sterols and fatty acids were identified. The metabolite profiling together with a suitable statistical analysis were used to discriminate between wild and cultured sea bass samples. Preliminary results show that discrimination between wild and cultured sea bass was obtained not only using fatty acid composition but also cholesterol and phosphatidylethanolamine and some water-soluble metabolites such as choline, trimethylamine oxide, glutamine, fumaric and malic acids.
核磁共振（NMR）技术被用作分析工具，以确定海鲈提取物的完整代谢谱：鉴定出了属于不同类别的水溶性代谢物，如糖类、氨基酸、二肽和有机酸，以及可溶于有机溶剂的代谢物，如脂质、甾醇和脂肪酸。代谢物谱分析以及适当的统计分析被用于区分野生和养殖的海鲈样本。初步结果表明，不仅利用脂肪酸组成，还利用胆固醇、磷脂酰乙醇胺以及一些水溶性代谢物（如胆碱、氧化三甲胺、谷氨酰胺、富马酸和苹果酸），能够区分野生和养殖的海鲈。
