Istituto di Metodologie Chimiche, Laboratorio di Risonanza Magnetica Annalaura Segre, CNR, Rome, Italy.
J Agric Food Chem. 2013 Feb 27;61(8):1718-26. doi: 10.1021/jf303248z. Epub 2012 Nov 8.
The metabolite profile of aqueous extracts of two peach varieties, Percoca Romagnola 7 and Flaminia, with different susceptibilities to Ceratitis capitata attack was investigated by means of 1D and 2D high-field NMR spectroscopy. Water-soluble metabolites belonging to different classes such as organic acids (citric, fumaric, malic, quinic, shikimic, and succinic acids), sugars (fucose, fructose, fructose-6-phosphate, glucose, glucose-6-phosphate, rhamnose, sucrose, and xylose), amino acids (alanine, asparagine, isoleucine, threonine, and valine) and other metabolites such as myo-inositol, choline, trigonelline, catechin, chlorogenic and neochlorogenic acids, orthophosphate, and α-l-glycerophosphorylcholine were identified. The metabolite profile together with a suitable statistical analysis was used to make a comparison between the two varieties. The levels of glucose, xylose, myo-inositol, choline, isoleucine, and valine were found to be higher in Flaminia than in Percoca Romagnola 7 samples, whereas the levels of fumaric acid, alanine, quinic acid, sucrose, fucose, and chlorogenic and neochlorogenic acid were found to be higher in Percoca Romagnola 7 than in Flaminia samples.
采用 1D 和 2D 高场 NMR 光谱法研究了两种桃品种（Percoca Romagnola 7 和 Flaminia）水提物的代谢轮廓，这两个品种对桃小食心虫的易感性不同。属于不同类别的水溶性代谢物，如有机酸（柠檬酸、富马酸、苹果酸、奎尼酸、莽草酸和琥珀酸）、糖（岩藻糖、果糖、果糖-6-磷酸、葡萄糖、葡萄糖-6-磷酸、鼠李糖、蔗糖和木糖）、氨基酸（丙氨酸、天冬酰胺、异亮氨酸、苏氨酸和缬氨酸）和其他代谢物，如肌醇、胆碱、三甲胺、儿茶素、绿原酸和新绿原酸、正磷酸盐和α-甘油磷酸胆碱被鉴定出来。代谢轮廓与适当的统计分析一起用于比较两个品种。发现 Flaminia 中的葡萄糖、木糖、肌醇、胆碱、异亮氨酸和缬氨酸的水平高于 Percoca Romagnola 7，而富马酸、丙氨酸、奎尼酸、蔗糖、岩藻糖和绿原酸和新绿原酸的水平高于 Percoca Romagnola 7。
