Food Technology, Faculty of Pharmacy, Lascaray Research Center, University of the Basque Country (UPV/EHU), Paseo de la Universidad No. 7, 01006 Vitoria, Spain.
Food Chem. 2012 Dec 1;135(3):1583-91. doi: 10.1016/j.foodchem.2012.06.002. Epub 2012 Jun 13.
Proton Nuclear Magnetic Resonance ((1)H NMR) spectroscopy was employed to study the lipids of farmed and wild European sea bass (Dicentrarchus labrax). This technique provided a great deal of detailed information on sea bass lipids composition and, once the spectra signals have been assigned, the simple observation of the spectra enables one to distinguish cultured from wild fish. This distinction is possible due to the presence in the former of high proportions of diunsaturated acyl groups, mainly linoleic. Furthermore, new approaches for quantitative characterization of sea bass lipids were developed. For the same season wild sea bass lipids contain not only higher molar percentages of omega-3 and of docosahexaenoic (DHA), but also higher concentrations of phosphatidylcholine and of cholesterol than farmed ones. However, in general, the absolute content of DHA and of eicosapentaenoic plus arachidonic (EPA+ARA) acyl groups as well as of phosphatidylcholine and cholesterol, were reasonably higher in farmed than in wild sea bass fillets, due to the higher total lipid content of the former. The presence of omega-1 acyl groups in all farmed samples and in some of the wild specimens was shown. As far as we know, this is the first time that omega-1 acyl groups have been identified and quantified in fish lipids by (1)H NMR.
采用质子核磁共振波谱((1)H NMR)技术研究养殖和野生欧洲海鲈(Dicentrarchus labrax)的脂质。该技术提供了有关海鲈脂质组成的大量详细信息，并且一旦对谱峰信号进行了分配，通过简单观察谱图就可以区分养殖和野生鱼类。这种区分是由于前者中二不饱和酰基（主要是亚油酸）的比例较高。此外，还开发了定量表征海鲈脂质的新方法。对于同一季节的野生海鲈，其脂质中的ω-3 和二十二碳六烯酸(DHA)不仅摩尔百分比更高，而且磷脂酰胆碱和胆固醇的浓度也更高。然而，一般来说，由于前者的总脂质含量较高，养殖海鲈鱼片的 DHA 和二十碳五烯酸加花生四烯酸(EPA+ARA)酰基以及磷脂酰胆碱和胆固醇的绝对含量要合理高一些。所有养殖样品和部分野生样本中均存在ω-1 酰基。据我们所知，这是首次通过(1)H NMR 鉴定和定量鱼类脂质中的 ω-1 酰基。
