Sen Biswarup, Chandra T S
Department of Chemistry, Indian Institute of Technology Madras, Chennai 600036, India.
Bioresour Technol. 2009 Jan;100(2):804-11. doi: 10.1016/j.biortech.2008.07.047. Epub 2008 Sep 19.
Two laboratory-scale systems were set up (i) composting (without earthworms) and (ii) vermicomposting (with earthworms) and were monitored for 60 days after pre-composting. The physico-chemical parameters (pH, C/N, organic matter, NH(4)(+)-N and ash content) showed similar evolution in both systems except a higher NH(4)(+)-N in the initial vermicomposts. However, principle component analysis (PCA) of enzymatic activities and community level physiological profiles revealed differences in the functional response of microbial communities in compost and vermicompost during maturation. Dehydrogenase activity and bacterial counts indicated a steady decrease in biological activity and population during composting, whereas vermicomposting exhibited higher activity on day 30 and a reduction in bacterial counts on day 10. PCA of denatured gradient gel electrophoresis (DGGE) profiles showed divergent dynamics of bacterial communities in two processes. These results indicated differences in the functional response and genetic structure of microbial community in composts and vermicomposts despite similar changes in their physico-chemical parameters.
（i）堆肥（无蚯蚓）和（ii）蚯蚓堆肥（有蚯蚓），并在预堆肥后监测60天。除了初始蚯蚓堆肥中较高的NH₄⁺-N外，两个系统中的物理化学参数（pH值、碳氮比、有机质、NH₄⁺-N和灰分含量）显示出相似的变化。然而，酶活性和群落水平生理剖面的主成分分析（PCA）揭示了堆肥和蚯蚓堆肥在成熟过程中微生物群落功能反应的差异。脱氢酶活性和细菌计数表明，堆肥过程中生物活性和种群数量稳步下降，而蚯蚓堆肥在第30天表现出较高的活性，细菌计数在第10天减少。变性梯度凝胶电泳（DGGE）图谱的PCA显示了两个过程中细菌群落的不同动态。这些结果表明，尽管堆肥和蚯蚓堆肥的物理化学参数有相似变化，但它们的微生物群落功能反应和遗传结构存在差异。
