神经元内注射鸟苷亚氨基二磷酸可模拟海兔的长期突触超极化。
Intraneuronal guanylyl-imidodiphosphate injection mimics long-term synaptic hyperpolarization in Aplysia.
作者信息
Treistman S N, Levitan I B
出版信息
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4689-92. doi: 10.1073/pnas.73.12.4689.
Abstract
The phosphodiesterase (3':5'-cyclic AMP 5'-nucleotidohydrolase, EC 3.1.4.17) inhibitor thepohylline enhances both the amplitude and duration of a long-lasting synaptic hyperpolarization in identified neuron R15 in Aplysia californica. Intraneuronal injection into R15 of glanylyl-imidodiphosphate, an adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activator, results in a deep and long-lasting hyperpolarization of the cell, similar to that produced by synaptic stimulation. Biochemical analysis confirms that guanylyl-imidodiphosphate activates adenylate cyclase in Aplysia californica nervous tissue, without affecting phosphodiesterase activity. These observations suggest that adenosine 3':5'-cyclic monophosphate plays a role in long-lasting synaptic inhibition and are consistent with a post-synaptic site of action for adenosine 3':5'-cyclic monophosphate.
摘要
磷酸二酯酶（3':5'-环磷酸腺苷5'-核苷酸水解酶，EC 3.1.4.17）抑制剂茶碱可增强加州海兔中已鉴定神经元R15的长时程突触超极化的幅度和持续时间。向R15神经元内注射腺苷酸环化酶[ATP焦磷酸裂解酶（环化），EC 4.6.1.1]激活剂鸟苷酰-亚氨基二磷酸，会导致细胞深度且持久的超极化，类似于突触刺激所产生的超极化。生化分析证实，鸟苷酰-亚氨基二磷酸可激活加州海兔神经组织中的腺苷酸环化酶，而不影响磷酸二酯酶的活性。这些观察结果表明，3':5'-环磷酸腺苷在长时程突触抑制中发挥作用，并且与3':5'-环磷酸腺苷的突触后作用位点一致。
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