章鱼胺和5-羟色胺刺激加州海兔腹神经节中特定蛋白质的磷酸化作用。
Octopamine- and serotonin-stimulated phosphorylation of specific protein in the abdominal ganglion of Aplysia californica.
作者信息
Levitan I B, Barondes S H
出版信息
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1145-8. doi: 10.1073/pnas.71.4.1145.
Abstract
Phosphorylation of a protein (or proteins) of molecular weight 120,000 in the Aplysia abdominal ganglion, as measured by incorporation of [(32)P] or [(33)P]sodium phosphate in vitro followed by separation of the phosphoproteins on sodium dodecyl sulfate-polyacrylamide gels, was specifically stimulated by incubation in the presence of the putative neurotransmitters octopamine or serotonin. The stimulatory effect of octopamine and serotonin was inhibited by phentolamine and methysergide, respectively, and was mimicked by incubation in the presence of dibutyryl cyclic AMP. Label-chase experiments indicated that the difference between control and octopamine-treated ganglia persists for several hours after removal of the drug from the incubation medium. This result suggests that neurotransmitters may produce relatively long-lasting changes in a phosphoprotein in the ganglion, perhaps in postsynaptic cells.
摘要
通过在体外掺入[（32）P]或[（33）P]磷酸钠，随后在十二烷基硫酸钠 - 聚丙烯酰胺凝胶上分离磷蛋白来测量，在海兔腹神经节中分子量为120,000的一种或多种蛋白质的磷酸化，在假定的神经递质章鱼胺或5-羟色胺存在下孵育可特异性地刺激。章鱼胺和5-羟色胺的刺激作用分别被酚妥拉明和甲基麦角新碱抑制，并且在二丁酰环磷酸腺苷存在下孵育可模拟这种作用。标记追踪实验表明，从孵育培养基中去除药物后，对照神经节和经章鱼胺处理的神经节之间的差异持续数小时。该结果表明神经递质可能在神经节中的磷蛋白中产生相对持久的变化，也许是在突触后细胞中。
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