一种检测寡克隆IgM带的灵敏且可重复的方法。
A sensitive and reproducible method for the detection of oligoclonal IgM bands.
作者信息
Villar L M, González-Porqué P, Masjuán J, Alvarez-Cermeño J C, Bootello A, Keir G
机构信息
Department of Immunology, Hospital Ramón y Cajal, Madrid, Spain.
出版信息
J Immunol Methods. 2001 Dec 1;258(1-2):151-5. doi: 10.1016/s0022-1759(01)00492-6.
We have developed a sensitive and specific isoelectrofocusing (IEF) method for the detection of oligoclonal (OGC) IgM in the cerebrospinal fluid (CSF) and sera. In this procedure, ampholytes, in the range pH 5-8, were used to improve the resolution, and serum was diluted in saline to avoid IgM precipitation. Samples were treated with 50 mM dithiothreitol (DTT) at pH 9.5 to reduce IgM and improve the migration of the protein. Using an anti-IgM labelled with alkaline phosphatase, the limit of detection was found to be 0.1 ng in 5 microl of sample.
我们开发了一种灵敏且特异的等电聚焦（IEF）方法，用于检测脑脊液（CSF）和血清中的寡克隆（OGC）IgM。在此过程中，使用pH值范围为5-8的两性电解质来提高分辨率，血清用生理盐水稀释以避免IgM沉淀。样品在pH 9.5下用50 mM二硫苏糖醇（DTT）处理以还原IgM并改善蛋白质的迁移。使用碱性磷酸酶标记的抗IgM，发现5微升样品中的检测限为0.1纳克。
Zotero 插件