Feng Xiaohai, Wu Bo, Shen Xiaobo, Xu Hong
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing 210009, China.
Sheng Wu Gong Cheng Xue Bao. 2008 Jun;24(6):1075-9.
The production of propionic acid by Propionibacterium freudenreichii CCTCC M207015 was investigated in a Fibrous-bed bioreactor (FBB). The FBB was constructed by packing spiral cotton fibrous and immobilized into a bioreactor. By applying this bioreactor to propionic acid fermentation, the propionic acid yield had a significant improvement and reached 20.41 g/L, compared with the cell-free culture of 14.58 g/L (40 g/L of glucose). At the same time, the glucose exhausting time decreased from 120 h to 60 h. Batch fermentations at various glucose concentrations were carried out with FBB. Based on the analysis of the time course of production, fed-batch fermentation was also applied to produce propionic acid with FBB, the maximal propionic acid yield reached 45.91 g/L, and the proportion of propionic acid to total acids was about 72.31%.
在纤维床生物反应器（FBB）中研究了费氏丙酸杆菌CCTCC M207015生产丙酸的情况。该纤维床生物反应器是通过填充螺旋棉纤维并固定在生物反应器中构建而成。将此生物反应器应用于丙酸发酵时，与无细胞培养（40 g/L葡萄糖时为14.58 g/L）相比，丙酸产量有显著提高，达到了20.41 g/L。同时，葡萄糖耗尽时间从120小时减少到了60小时。使用纤维床生物反应器在不同葡萄糖浓度下进行了分批发酵。基于生产时间进程分析，还采用补料分批发酵用纤维床生物反应器生产丙酸，丙酸最大产量达到45.91 g/L，丙酸占总酸的比例约为72.31%。
