[假单胞菌属对石油的脱氮作用]

[Denitrogenation of petroleum by a Pseudomonas sp].

作者信息

Li Li, Xu Ping, Shi Quan

机构信息

State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2008 Jun;24(6):1080-4.

PMID:18807996

Abstract

Biodenitrogenation of petroleum oil was investigated by a previously isolated carbazole-degrader Pseudomonas sp. XLDN4-9. In a tetradecane-aqueous phase system, biodegradation of carbazole was enhanced by the presence of n-tetradecane. And strain XLDN4-9 was capable of absorbing 95.2% of 2 g/L carbazole dissolved in diesel within 15 hours. Significant denitrogentation of crude oil, diesel and lubricanting oil was detected by strain XLDN4-9. Removal of carbazole, methylcarbazole, and dimethylcarbazole in diesel was confirmed by using GC-MS. After 3 days, 99% of carbazole and 15% of dimethyl carbazole was degraded. And the removal rate of 1-, 2-, 3-, and 4-methyl carbazole was determined to be 63.4%, 87.6%, 78.4%, and 66.5% respectively.

摘要

利用先前分离得到的咔唑降解菌假单胞菌属XLDN4 - 9对石油进行生物脱氮研究。在十四烷 - 水相体系中，正十四烷的存在促进了咔唑的生物降解。菌株XLDN4 - 9能够在15小时内吸收溶解于柴油中的2 g/L咔唑的95.2%。菌株XLDN4 - 9对原油、柴油和润滑油具有显著的脱氮作用。通过气相色谱 - 质谱联用仪（GC - MS）确认了柴油中咔唑、甲基咔唑和二甲基咔唑的去除情况。3天后，99%的咔唑和15%的二甲基咔唑被降解。并且测定1 - 甲基咔唑、2 - 甲基咔唑、3 - 甲基咔唑和4 - 甲基咔唑的去除率分别为63.4%、87.6%、78.4%和66.5%。