Calcutta School of Tropical Medicine, Kolkata, India.
Appl Biochem Biotechnol. 2012 Dec;168(7):1765-77. doi: 10.1007/s12010-012-9895-1. Epub 2012 Sep 18.
A pure colony of a bacterium from contaminated soil was isolated by exploiting 3,6-dimethyl-1-keto-1,2,3,4-tetrahydrocarbazole, a novel carbazole derivative, having indole moiety as well as 3-methyl functionality both in aromatic and hydro-aromatic moiety, as a sole source of carbon and energy. Taxonomical studies, biochemical analysis, and 16S rDNA sequence analysis indicated that the isolated strain has close similarity with Pseudomonas sp. Thin-layer chromatography followed by HPLC and mass spectroscopic study indicates that the isolated Pseudomonas sp. STM 997 degrades 3,6-dimethyl-1-keto-1,2,3,4-tetrahydrocarbazole, and this strain may be useful in the bioremediation of environments contaminated by the compounds containing carbazole moiety with methyl substituents at various reactive sites. This study also provides an evidence in favor of the suggested biodegradation of 3-methylcarbazole to carbazole in plants.
从污染土壤中分离出的一种纯细菌菌落在利用 3,6-二甲基-1-酮-1,2,3,4-四氢咔唑作为唯一的碳源和能源时，该化合物具有吲哚部分以及在芳族和杂芳族部分中均带有 3-甲基官能团的新型咔唑衍生物的情况下被分离出来。分类学研究、生化分析和 16S rDNA 序列分析表明，分离出的菌株与假单胞菌属具有密切的相似性。薄层层析（TLC）随后的 HPLC 和质谱研究表明，分离出的假单胞菌属 STM 997 可降解 3,6-二甲基-1-酮-1,2,3,4-四氢咔唑，该菌株可能有助于生物修复含有咔唑部分且在各种反应部位带有甲基取代基的化合物污染的环境。这项研究也为植物中 3-甲基咔唑向咔唑的生物降解提供了证据。
