Inohara Hidenori, Segawa Tatsuya, Miyauchi Akira, Yoshii Tadashi, Nakahara Susumu, Raz Avraham, Maeda Masahiro, Miyoshi Eiji, Kinoshita Noriaki, Yoshida Hiroshi, Furukawa Masashi, Takenaka Yukinori, Takamura Yuki, Ito Yasuhiro, Taniguchi Naoyuki
Department of Otolaryngology, Osaka University School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
Biochem Biophys Res Commun. 2008 Nov 21;376(3):605-10. doi: 10.1016/j.bbrc.2008.09.041. Epub 2008 Sep 20.
In order to address whether galectin-3 in the sera and fine needle aspirates serve as a diagnostic marker distinguishing between benign and malignant thyroid nodules, we developed an enzyme-linked immunosorbent assay. We quantified galectin-3 in fine needle aspirates from a series of 118 patients with thyroid nodules and serum galectin-3 from another series of 46 patients, which were compared with final histology after thyroidectomy. Relative galectin-3 value (ng/mg), defined as galectin-3 concentration (ng/ml) divided by total protein concentration (mg/ml) in fine needle aspirates, was significantly higher in papillary carcinoma than in the other thyroid entities. There was no significant difference in serum galectin-3 level among patients with thyroid nodules and healthy individuals. Accordingly, relative galectin-3 value allows a definitive diagnosis of papillary carcinoma independent of cellular morphology, whereas serum galectin-3 does not serve as a marker for papillary carcinoma.
为了探讨血清和细针穿刺抽吸物中的半乳糖凝集素-3是否可作为区分甲状腺良恶性结节的诊断标志物，我们开发了一种酶联免疫吸附测定法。我们对一系列118例甲状腺结节患者的细针穿刺抽吸物中的半乳糖凝集素-3以及另一系列46例患者的血清半乳糖凝集素-3进行了定量，并将其与甲状腺切除术后的最终组织学结果进行比较。细针穿刺抽吸物中的半乳糖凝集素-3相对值（ng/mg）定义为半乳糖凝集素-3浓度（ng/ml）除以总蛋白浓度（mg/ml），在乳头状癌中显著高于其他甲状腺疾病。甲状腺结节患者和健康个体的血清半乳糖凝集素-3水平无显著差异。因此，半乳糖凝集素-3相对值可独立于细胞形态对乳头状癌做出明确诊断，而血清半乳糖凝集素-3不能作为乳头状癌的标志物。
