Schepers Koen, Swart Erwin, van Heijst Jeroen W J, Gerlach Carmen, Castrucci Maria, Sie Daoud, Heimerikx Mike, Velds Arno, Kerkhoven Ron M, Arens Ramon, Schumacher Ton N M
Division of Immunology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
J Exp Med. 2008 Sep 29;205(10):2309-18. doi: 10.1084/jem.20072462. Epub 2008 Sep 22.
T cells, as well as other cell types, are composed of phenotypically and functionally distinct subsets. However, for many of these populations it is unclear whether they develop from common or separate progenitors. To address such issues, we developed a novel approach, termed cellular barcoding, that allows the dissection of lineage relationships. We demonstrate that the labeling of cells with unique identifiers coupled to a microarray-based detection system can be used to analyze family relationships between the progeny of such cells. To exemplify the potential of this technique, we studied migration patterns of families of antigen-specific CD8(+) T cells in vivo. We demonstrate that progeny of individual T cells rapidly seed independent lymph nodes and that antigen-specific CD8(+) T cells present at different effector sites are largely derived from a common pool of precursors. These data show how locally primed T cells disperse and provide a technology for kinship analysis with wider utility.
T细胞以及其他细胞类型，由表型和功能上不同的亚群组成。然而，对于许多这些细胞群体，尚不清楚它们是由共同的还是分开的祖细胞发育而来。为了解决此类问题，我们开发了一种名为细胞条形码的新方法，该方法可用于剖析谱系关系。我们证明，用与基于微阵列的检测系统偶联的独特标识符对细胞进行标记，可用于分析此类细胞后代之间的家族关系。为了举例说明该技术的潜力，我们研究了体内抗原特异性CD8(+) T细胞家族的迁移模式。我们证明，单个T细胞的后代迅速在独立的淋巴结中定植，并且存在于不同效应位点的抗原特异性CD8(+) T细胞在很大程度上源自共同的前体细胞池。这些数据展示了局部启动的T细胞如何分散，并提供了一种具有更广泛用途的亲缘关系分析技术。
