Askary Amjad, Chen Wei, Choi Junhong, Du Lucia Y, Elowitz Michael B, Gagnon James A, Schier Alexander F, Seidel Sophie, Shendure Jay, Stadler Tanja, Tran Martin
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA.
Department of Genome Sciences, University of Washington, Seattle, WA, USA.
Nat Rev Genet. 2025 Mar;26(3):203-222. doi: 10.1038/s41576-024-00788-w. Epub 2024 Nov 25.
A paradigm for biology is emerging in which cells can be genetically programmed to write their histories into their own genomes. These records can subsequently be read, and the cellular histories reconstructed, which for each cell could include a record of its lineage relationships, extrinsic influences, internal states and physical locations, over time. DNA recording has the potential to transform the way that we study developmental and disease processes. Recent advances in genome engineering are driving the development of systems for DNA recording, and meanwhile single-cell and spatial omics technologies increasingly enable the recovery of the recorded information. Combined with advances in computational and phylogenetic inference algorithms, the DNA recording paradigm is beginning to bear fruit. In this Perspective, we explore the rationale and technical basis of DNA recording, what aspects of cellular biology might be recorded and how, and the types of discovery that we anticipate this paradigm will enable.
一种生物学范式正在兴起，在这种范式中，细胞可以通过基因编程将其历史记录在自身的基因组中。这些记录随后可以被读取，并重建细胞历史，随着时间的推移，对于每个细胞来说，这可能包括其谱系关系、外部影响、内部状态和物理位置的记录。DNA记录有潜力改变我们研究发育和疾病过程的方式。基因组工程的最新进展推动了DNA记录系统的发展，与此同时，单细胞和空间组学技术越来越能够恢复记录的信息。结合计算和系统发育推断算法的进展，DNA记录范式开始结出硕果。在这篇观点文章中，我们探讨了DNA记录的基本原理和技术基础、细胞生物学的哪些方面可能被记录以及如何记录，以及我们预计这种范式将带来的发现类型。
