Li Jian-hua, Song Da-yong, Xu Yong-gang, Huang Zheng, Yue Wu
Department of Neurosurgery, The Fourth College Hospital of Haerbin Medical University, Harbin, China.
Neurol Sci. 2008 Sep;29(4):229-35. doi: 10.1007/s10072-008-0972-8. Epub 2008 Sep 20.
To study the cytotoxicity induced by haematoporphyrin monomethyl ether (HMME)-mediated sonodynamic therapy (SDT) on C6 glioma cells.
The potent photosensitizer HMME was used as the sensitizer. Rat C6 glioma cells were incubated with HMME (10 microg/mL) in the dark for 2 h and then subjected to ultrasound treatment at 1.0 MHz and 0.5 W/cm2 for 2 min. The growth inhibition rate at different time points after SDT was determined by MTT assay. The apoptotic rate and cell circle profiles were examined with flow cytometry. Fine structures were observed with transmission electron microscope (TEM). The sonodynamic effect on the glioma cells was also studied in the absence or presence of various reactive oxygen species (ROS) scavengers.
The growth inhibition rate of C6 glioma cells after SDT significantly increased. SDT also increased the apoptosis and proliferation rate (APR). TEM examination showed the morphological features of apoptosis or necrosis. The addition of NaN(3) showed a strong protective effect again SDT.
Our data indicated that SDT could kill C6 glioma cells in vitro and possibility through induction of apoptosis and necrosis. Singlet oxygen ((1)O2) may play an important role in SDT.
研究血卟啉单甲醚（HMME）介导的声动力疗法（SDT）对C6胶质瘤细胞的细胞毒性。
使用强效光敏剂HMME作为敏化剂。将大鼠C6胶质瘤细胞在黑暗中与HMME（10微克/毫升）孵育2小时，然后在1.0兆赫和0.5瓦/平方厘米的条件下进行超声处理2分钟。通过MTT法测定SDT后不同时间点的生长抑制率。用流式细胞术检测凋亡率和细胞周期分布。用透射电子显微镜（TEM）观察精细结构。还在存在或不存在各种活性氧（ROS）清除剂的情况下研究了声动力对胶质瘤细胞的作用。
SDT后C6胶质瘤细胞的生长抑制率显著增加。SDT还增加了凋亡和增殖率（APR）。TEM检查显示了凋亡或坏死的形态学特征。添加NaN(3)对SDT显示出很强的保护作用。
我们的数据表明，SDT可以在体外杀死C6胶质瘤细胞，可能是通过诱导凋亡和坏死。单线态氧((1)O2)可能在SDT中起重要作用。
