Truman J W, Fallon A M, Wyatt G R
Science. 1976 Dec 24;194(4272):1432-4. doi: 10.1126/science.188130.
The eclosion hormone triggers a stereotyped preprogrammed pattern of behavior in silk moths. The effects of the hormone were duplicated by the injection of dibutyryl adenosine 3', 5'-monophosphate, adenosine 3', 5'-monophosphate (cyclic AMP), or guanosine 3', 5'-monophosphate (cyclic GMP) into theophylline-treated pharate moths. Treatment with theophylline reduced the latency of the response to a low dose of hormone, presumably by blocking phosphodiesterase. Endogenous levels of cyclic AMP, but not cyclic GMP, increased significantly in the central nervous system within 10 minutes after hormone injection. We conclude that an early step leading to the release of the eclosion motor program is an increase in cyclic AMP in target neurons of the central nervous system.
羽化激素触发蚕蛾中一种刻板的预编程行为模式。通过向经茶碱处理的化蛹蛾注射二丁酰腺苷3',5'-单磷酸、腺苷3',5'-单磷酸（环磷酸腺苷）或鸟苷3',5'-单磷酸（环磷酸鸟苷），可复制该激素的作用。用茶碱处理可缩短对低剂量激素反应的潜伏期，推测是通过阻断磷酸二酯酶来实现的。激素注射后10分钟内，中枢神经系统内的环磷酸腺苷水平显著升高，而环磷酸鸟苷水平未升高。我们得出结论，导致羽化运动程序释放的早期步骤是中枢神经系统靶神经元中环磷酸腺苷的增加。
