Zannier A, Drancourt M, Franceschi J P, Aubaniac J M, Raoult D
Laboratoire de Microbiologie, Hôpital de la Conception, Marseille.
Pathol Biol (Paris). 1991 May;39(5):543-6.
The purpose of this work is to compare, a conventional technique, for bacterial isolation, with a lysis-centrifugation method using a rapid freezing in liquid nitrogen, followed by decongelation at 37 degrees C for bone and joint samples. The bone and joint specimens were biopsies and punctions (35 cases) or fistula (10 cases). The residual antibiotic activity of the sample was determined using a susceptible strain of Micrococcus luteus and of Staphylococcus epidermidis. Among the 45 samples, 20 were sterile with both methods among which 8 exhibited a residual antibiotic activity which may have contributed to isolation failure. In 12 cases, bacterial isolates were obtained with both methods. Cultures were obtained from 13 samples with the lysis centrifugation method alone. The isolates were 4 Staphylococcus aureus, 8 coagulase-negative Staphylococcus and 1 Acinetobacter sp. These results suggest that the intracellular location of bacteria may be responsible for the negative cultures from bone or joint specimens.
这项工作的目的是将一种用于细菌分离的传统技术与一种采用液氮快速冷冻、随后在37摄氏度解冻的裂解离心法进行比较，用于骨和关节样本。骨和关节标本包括活检和穿刺样本（35例）或瘘管样本（10例）。使用藤黄微球菌和表皮葡萄球菌的敏感菌株测定样本的残留抗生素活性。在45个样本中，两种方法均显示20个样本无菌，其中8个样本具有残留抗生素活性，这可能导致了分离失败。在12个病例中，两种方法均获得了细菌分离株。仅通过裂解离心法从13个样本中获得了培养物。分离出的菌株包括4株金黄色葡萄球菌、8株凝固酶阴性葡萄球菌和1株不动杆菌属。这些结果表明，细菌的细胞内定位可能是导致骨或关节标本培养阴性的原因。
