Tkaczyk Eric R, Mauring Koit, Tkaczyk Alan H, Krasnenko Veera, Ye Jing Yong, Baker James R, Norris Theodore B
Center for Ultrafast Optical Science, University of Michigan, 2200 Bonisteel Boulevard, Ann Arbor, MI 48109-2099, USA.
Biochem Biophys Res Commun. 2008 Nov 28;376(4):733-7. doi: 10.1016/j.bbrc.2008.09.075. Epub 2008 Sep 24.
We demonstrate optical coherent control of the two-photon fluorescence of the blue fluorescent protein (BFP), which is of interest in investigations of protein-protein interactions. In addition to biological relevance, BFP represents an interesting target for coherent control from a chemical perspective due to its many components of highly nonexponential fluorescence decay and low quantum yield resulting from excited state isomerization. Using a genetic algorithm with a multiplicative (rather than ratiometric) fitness parameter, we are able to control the ratio of BFP fluorescence to second-harmonic generation without a considerable drop in the maximized signal. The importance of linear chirp and power-scaling on the discrimination process is investigated in detail.
我们展示了对蓝色荧光蛋白（BFP）双光子荧光的光学相干控制，这在蛋白质 - 蛋白质相互作用研究中具有重要意义。除了生物学相关性外，从化学角度来看，BFP也是相干控制的一个有趣目标，因为它具有许多高度非指数荧光衰减的成分，并且由于激发态异构化导致量子产率较低。使用具有乘法（而非比例）适应度参数的遗传算法，我们能够控制BFP荧光与二次谐波产生的比率，同时最大化信号不会有显著下降。详细研究了线性啁啾和功率缩放对鉴别过程的重要性。
