Liu Congyue, Liu Wei, Wang Shufeng, Li Hongjia, Lv Zhilong, Zhang Fa, Zhang Donghui, Teng Junlin, Zheng Tao, Li Donghai, Zhang Mingshu, Xu Pingyong, Gong Qihuang
State Key Laboratory for Artificial Microstructure and Mesoscopic Physics, Department of Physics, Peking University, Beijing 100871, China.
Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan 030006, Shanxi, China.
Sci Adv. 2020 Apr 17;6(16):eaaw6579. doi: 10.1126/sciadv.aaw6579. eCollection 2020 Apr.
Super-resolution nanoscopy based on wide-field microscopic imaging provided high efficiency but limited resolution. Here, we demonstrate a general strategy to push its resolution down to ~50 nm, which is close to the range of single molecular localization microscopy, without sacrificing the wide-field imaging advantage. It is done by actively and simultaneously modulating the characteristic emission of each individual emitter at high density. This method is based on the principle of excited state coherent control on single-particle two-photon fluorescence. In addition, the modulation efficiently suppresses the noise for imaging. The capability of the method is verified both in simulation and in experiments on ZnCdS quantum dot-labeled films and COS7 cells. The principle of coherent control is generally applicable to single-multiphoton imaging and various probes.
基于宽场显微成像的超分辨率纳米显微镜具有高效率但分辨率有限。在此，我们展示了一种通用策略，可在不牺牲宽场成像优势的情况下，将其分辨率降低至约50纳米，这接近单分子定位显微镜的分辨率范围。这是通过在高密度下主动且同时调制每个单独发射体的特征发射来实现的。该方法基于单粒子双光子荧光的激发态相干控制原理。此外，这种调制有效地抑制了成像噪声。该方法的能力在模拟以及对硫化锌镉量子点标记薄膜和COS7细胞的实验中均得到了验证。相干控制原理普遍适用于单多光子成像和各种探针。
