Albert D J, Jonik R H, Walsh M L
Psychology Department, University of British Columbia, Vancouver, Canada.
Physiol Behav. 1991 Apr;49(4):673-7. doi: 10.1016/0031-9384(91)90300-d.
Female rats were individually housed with a sterile male for the duration of the experiment. Beginning 7 to 10 weeks after the start of cohabitation, each female was tested for aggression toward an unfamiliar female at weekly intervals for 3 weeks. Females that displayed consistent and substantial aggression were given one of the following treatments: ovariectomy followed by both testosterone and estradiol implants, ovariectomy followed by 2 empty implants, or sham ovariectomy followed by 2 empty implants. The implants were subcutaneously placed hormone-filled Silastic capsules. They were expected to produce a serum testosterone concentration of 0.5 ng/ml and an estradiol concentration of 15 pg/ml. Postoperatively, the aggression of each female continued to be assessed on a weekly basis for 3 weeks. Ovariectomized females with hormone implants displayed a level of aggression postoperatively similar to that of sham-ovariectomized females and significantly greater than that of ovariectomized females with empty implants. These results, together with others, suggest that estradiol and testosterone act together to form the hormonal foundation of hormone-dependent aggression by females cohabiting with a sterile male.
在整个实验过程中，将雌性大鼠与一只无菌雄性单独饲养在一起。从同居开始7至10周后，每周对每只雌性大鼠进行一次针对陌生雌性的攻击性测试，持续3周。表现出持续且强烈攻击性的雌性大鼠接受以下处理之一：卵巢切除术后植入睾酮和雌二醇，卵巢切除术后植入2个空植入物，或假卵巢切除术后植入2个空植入物。植入物是皮下放置的装有激素的硅橡胶胶囊。预期它们能使血清睾酮浓度达到0.5 ng/ml，雌二醇浓度达到15 pg/ml。术后，每周对每只雌性大鼠的攻击性持续评估3周。植入激素的卵巢切除雌性大鼠术后的攻击性水平与假卵巢切除雌性大鼠相似，且显著高于植入空植入物的卵巢切除雌性大鼠。这些结果与其他结果一起表明，雌二醇和睾酮共同作用，为与无菌雄性同居的雌性激素依赖性攻击行为形成激素基础。
