Stjärne P, Lacroix J S, Anggård A, Lundberg J M
Department of Oto-Rhino-Laryngology, Karolinska Hospital, Stockholm, Sweden.
Regul Pept. 1991 May 17;33(3):251-62. doi: 10.1016/0167-0115(91)90228-9.
The overflow of calcitonin gene-related peptide like-immunoreactivity (CGRP-LI) in the nasal venous effluent upon antidromic stimulation of the maxillary division of the trigeminal nerve with 6.9 Hz for 3 min or upon capsaicin (0.3 mumol bolus injection) were analysed in the nasal mucosa of sympathectomized pentobarbital anaesthetized pigs. The overflow of CGRP-LI upon antidromic stimulation displayed a slower appearance in the venous effluent than the overflow upon bolus injection of capsaicin. The vascular effects as revealed by the arterial blood flow, the venous blood flow, the blood volume of the nasal mucosa, i.e., the filling of the capacitance vessels and the superficial mucosal blood flow as revealed by the laser-Doppler signal were also studied. Antidromic stimulation of the trigeminal nerve as well as capsaicin bolus injection induced a marked vasodilation which was parallel to the overflow of CGRP. However, capsaicin bolus injection also resulted in a marked increase in the mean arterial blood pressure which may be due to reflex activation of sympathetic fibers. In conclusion, we have demonstrated that chemical stimulation with capsaicin as well as antidromic stimulation of nasal sensory nerves in sympathectomized animals induces both vasodilation and overflow of CGRP-LI in vivo. This indicates that CGRP may contribute to the sensory regulation of the microcirculation in the nasal mucosa.
在戊巴比妥麻醉的去交感神经猪的鼻粘膜中，分析了用6.9Hz的频率对三叉神经上颌支进行3分钟逆向刺激或给予辣椒素（0.3μmol推注）后鼻静脉流出物中降钙素基因相关肽样免疫反应性（CGRP-LI）的溢出情况。逆向刺激时CGRP-LI的溢出在静脉流出物中出现的速度比推注辣椒素后的溢出要慢。还研究了动脉血流、静脉血流、鼻粘膜血容量（即容量血管的充盈情况）以及激光多普勒信号显示的浅表粘膜血流所揭示的血管效应。对三叉神经的逆向刺激以及辣椒素推注均引起明显的血管舒张，这与CGRP的溢出平行。然而，辣椒素推注还导致平均动脉血压显著升高，这可能是由于交感神经纤维的反射性激活所致。总之，我们已经证明，在去交感神经的动物中，用辣椒素进行化学刺激以及对鼻感觉神经进行逆向刺激均可在体内诱导血管舒张和CGRP-LI的溢出。这表明CGRP可能有助于鼻粘膜微循环的感觉调节。
