Chen Shi-Lin, Sun Yong-Qiao, Song Jing-Yuan, Li Ying, Li Chen-Ji, Hu Song-Nian, Li Xi-Wen, Yao Hui, Zhang Xiao-Wei
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100094, China.
Yao Xue Xue Bao. 2008 Jun;43(6):657-63.
To investigate the profile of gene expression in American ginseng (Panax quinquefolium L.) and discover its functional genes, for the first time, expressed sequence tags (EST) library of four-year-old American ginseng roots has been established. According to BLAST and Gene Ontology analysis, eleven genes, encoding cytochrome P450, glucosyltransferase, farnesyltransferase and cyclase family protein, are found to be associated with ginsenosides biosynthesis. Six other genes are obtained encoding auxin-regulated protein, auxin response factor 4 and auxin-repressed protein in the roots of American ginseng. In addition, thirteen expressed transcripts are stress-connected proteins and twelve expressed other transcripts are closely related to plant defense in four-year-old American ginseng roots. Furthermore, 62 genes no hit in BLAST and in Interproscan may be new genes. These results indicate EST is an useful tool for research on functional genomics of P. quinquefolium and it can be applied to the molecular modification of the ginsenosides biosynthetic pathway ultimately for improving the quality of American ginseng germplasm.
为了研究西洋参（Panax quinquefolium L.）的基因表达谱并发现其功能基因，首次构建了四年生西洋参根的表达序列标签（EST）文库。根据BLAST和基因本体分析，发现11个编码细胞色素P450、糖基转移酶、法尼基转移酶和环化酶家族蛋白的基因与人参皂苷生物合成相关。在西洋参根中还获得了另外6个编码生长素调节蛋白、生长素响应因子4和生长素抑制蛋白的基因。此外，在四年生西洋参根中，13个表达转录本是胁迫相关蛋白，12个表达的其他转录本与植物防御密切相关。此外，62个在BLAST和Interproscan中无匹配的基因可能是新基因。这些结果表明，EST是西洋参功能基因组学研究的有用工具，最终可应用于人参皂苷生物合成途径的分子修饰，以提高西洋参种质质量。
