Bozin Biljana, Mimica-Dukic Neda, Bogavac Mirjana, Suvajdzic Ljiljana, Simin Natasa, Samojlik Isidora, Couladis Maria
Faculty of Medicine, Department of Pharmacy, Hajduk Veljkova 3, 21 000 Novi Sad, Serbia.
Molecules. 2008 Sep 2;13(9):2058-68. doi: 10.3390/molecules13092058.
The in vitro antioxidant and antimicrobial activities of two Achillea millefolium (Adanson) Koch s.l species essential oils (A. collina Becker ex Heimerl s.l. and A. pannonica Scheele, Asteraceae) originating from the Golija and Radan mountains (Serbia) were investigated. The chemical profiles of the essential oils were evaluated by GC-MS. Antioxidant activity was assessed as free radical scavenging capacity (RSC) towards 2,2-diphenyl-1-picrylhydrazil (DPPH) radicals, together with effects on lipid peroxidation (LP). Antibacterial activity was examined on 21 bacterial strains. Based on the chemical composition of the essential oil, A. collina s.l. from Mount Golija was classified as a chamazulene chemotype (tetraploid). The high percentage of oxygenated monoterpenes and absence of azulene in the essential oil obtained from A. pannonica from Radan pointing that this population is octaploid. Essential oil of A. pannonica expressed stronger antimicrobial activity on almost all tested bacteria. Furthermore, this essential oil expressed higher scavenging effects on DPPH radical (IC(50) = 0.52 comparing to 0.62 mug/mL). Only in the LP evaluation, essential oil of A. collina s.l. from Golija exhibited stronger antioxidant activity (IC(50) = 0.75 comparing to 2.12 mug/mL).
对源自塞尔维亚戈利贾山和拉丹山的两种千叶蓍（菊科）精油（戈利贾千叶蓍和潘诺尼亚千叶蓍）的体外抗氧化和抗菌活性进行了研究。通过气相色谱 - 质谱联用仪（GC - MS）对精油的化学组成进行了评估。抗氧化活性通过对2,2 - 二苯基 - 1 - 苦基肼（DPPH）自由基的自由基清除能力（RSC）以及对脂质过氧化（LP）的影响来评估。对21种细菌菌株进行了抗菌活性检测。基于精油的化学成分，来自戈利贾山的戈利贾千叶蓍被归类为天蓝烃化学型（四倍体）。从拉丹山的潘诺尼亚千叶蓍获得的精油中氧化单萜的高比例和天蓝烃的缺失表明该种群是八倍体。潘诺尼亚千叶蓍的精油对几乎所有测试细菌都表现出更强的抗菌活性。此外，这种精油对DPPH自由基表现出更高的清除效果（IC(50)=0.52，而另一种为0.62μg/mL）。仅在脂质过氧化评估中，来自戈利贾山的戈利贾千叶蓍精油表现出更强的抗氧化活性（IC(50)=0.75，而另一种为2.12μg/mL）。
