a Antibiotics, Antifungal: Physical Chemistry, Synthesis and Biological Activity Laboratory, Department of Biology, Faculty of Natural Sciences and Life Sciences and Earth and the Universe , University of Tlemcen , P.O. Box 119, Tlemcen 13000 , Algeria.
Nat Prod Res. 2014;28(11):795-807. doi: 10.1080/14786419.2014.883392. Epub 2014 Feb 24.
This study describes the chemical composition and the antibacterial, antifungal and antioxidant activities of the essential oil extracted from aerial parts of the Algerian Eryngium tricuspidatum L., obtained by hydrodistillation and analysed by using the combination of gas chromatography (GC) and GC/mass spectrometry. A total of 63 compounds were identified accounting for 93.1% of the total oil. Chemical composition of oil was characterised by a high proportion of oxygenated sesquiterpenes (49.6%) among which α-bisabolol (32.6%) was the predominant compound. The sesquiterpene hydrocarbons represent the second major fraction (31.9%) with α-curcumene (6.5%) being the predominant one. Antibacterial and antifungal activities of the oil were tested using the micro-well determination of minimum inhibitory concentration (MIC) assay against eleven bacteria and two Candida species. It was found that the aerial parts of E. tricuspidatum exhibited interesting antibacterial and anticandidal activities (MIC = 9 μg/mL against several strains of bacteria and MIC = 4.6 μg/mL against Candida albicans). The antioxidant effect of this oil was evaluated using the 2,2-diphenyl-l-1-picrylhydrazil (DPPH) and ferric reducing antioxidant power (FRAP) assays. Results revealed significant activities (DPPH method: IC₅₀ = 510 μg/mL; FRAP assay: reducing power of oil increases from 0.0188 at 5 μg/mL to 0.5016 at 1000 μg/mL).
本研究描述了从阿尔及利亚刺芹（Eryngium tricuspidatum L.）地上部分提取的精油的化学成分以及其抗菌、抗真菌和抗氧化活性，该精油通过水蒸馏获得，并使用气相色谱（GC）和 GC/质谱联用进行分析。共鉴定出 63 种化合物，占总油的 93.1%。油的化学成分以含氧倍半萜（49.6%）为主，其中 α-毕澄茄醇（32.6%）为主要化合物。倍半萜烃代表第二大主要成分（31.9%），其中 α-姜黄烯（6.5%）为主要化合物。使用微量稀释法测定最小抑菌浓度（MIC）测定法对 11 种细菌和 2 种念珠菌对油的抗菌和抗真菌活性进行了测试。结果发现，刺芹的地上部分表现出有趣的抗菌和抗假丝酵母活性（对几种细菌的 MIC = 9 μg/mL，对白色念珠菌的 MIC = 4.6 μg/mL）。通过 2,2-二苯基-1-苦基肼（DPPH）和铁还原抗氧化能力（FRAP）测定法评估了这种油的抗氧化作用。结果表明，其具有显著的活性（DPPH 法：IC₅₀ = 510 μg/mL；FRAP 法：油的还原力从 5 μg/mL 时的 0.0188 增加到 1000 μg/mL 时的 0.5016）。
