Ma Zhong-Ze, Xu Wei, Jensen Niels H, Roth Bryan L, Liu-Chen Lee-Yuan, Lee David Y W
Bio-Organic and Natural Products Laboratory, McLean Hospital, Harvard Medical School, 115 Mill Street, Belmont, MA 02478, USA.
Molecules. 2008 Sep 25;13(9):2303-12. doi: 10.3390/molecules13092303.
Bioactivity-guided fractionation of Corydalis yanhusuo has resulted in the isolation of eight known isoquinoline alkaloids - tetrahydropalmatine, isocorypalmine, stylopine, corydaline, columbamine, coptisin, 13-methylpalmatine, and dehydrocorybulbine. The tertiary alkaloids were further analyzed by chiral HPLC to determine the ratios of d-and l-isomers. The isolated compounds were screened for their binding affinities at the dopamine D(1) receptor. Isocorypalmine had the highest affinity (K(i) = 83 nM). The structure-affinity relationships of these alkaloids are discussed.
对延胡索进行生物活性导向的分离，已分离出8种已知的异喹啉生物碱——四氢巴马汀、异紫堇杷明碱、斯氏紫堇碱、紫堇灵、黄连碱、黄连次碱、13-甲基巴马汀和去氢紫堇碱。通过手性高效液相色谱法对叔生物碱进一步分析，以确定d-异构体和l-异构体的比例。对分离出的化合物进行多巴胺D(1)受体结合亲和力筛选。异紫堇杷明碱具有最高亲和力（抑制常数K(i)=83 nM）。并讨论了这些生物碱的结构-亲和力关系。
