黄芪多糖的分子量及单糖组成
Molecular weight and monosaccharide composition of Astragalus polysaccharides.
作者信息
Xu Du-Juan, Xia Quan, Wang Jia-Jia, Wang Pei-Pei
机构信息
Department of Pharmacy, First Affiliated Hospital of Anhui Medical University, Hefei, 230022. PR China.
出版信息
Molecules. 2008 Oct 1;13(10):2408-15. doi: 10.3390/molecules13102408.
Two polysaccharides (APS-I and APS-II) were isolated from the water extract of Radix Astragali and purified through ethanol precipitation, deproteination and by ion-exchange and gel-filtration chromatography. Their molecular weight was determined using high performance liquid chromatography and gel permeation chromatography (HPLC-GPC) and their monosaccharide composition was analyzed by TLC and HPLC methods, using a refractive index detector (RID) and an NH(2) column. It was shown that APS-I consisted of arabinose and glucose and APS-II consisted of rhamnose, arabinose and glucose, in a molar ratio of 1:3.45 and 1:6.25:17.86, respectively. The molecular weights (Mw) of APS-I and APS-II were 1,699,100 Da and 1,197,600 Da, respectively.
从黄芪水提取物中分离出两种多糖（APS-I和APS-II），并通过乙醇沉淀、脱蛋白以及离子交换和凝胶过滤色谱法进行纯化。使用高效液相色谱和凝胶渗透色谱（HPLC-GPC）测定其分子量，并通过薄层色谱（TLC）和高效液相色谱（HPLC）方法，使用示差折光检测器（RID）和氨基柱分析其单糖组成。结果表明，APS-I由阿拉伯糖和葡萄糖组成，APS-II由鼠李糖、阿拉伯糖和葡萄糖组成，摩尔比分别为1:3.45和1:6.25:17.86。APS-I和APS-II的分子量（Mw）分别为1,699,100 Da和1,197,600 Da。
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