Sharpe Martyn A, Ferguson-Miller Shelagh
Department of Neurosurgery, The Methodist Hospital, Houston, TX 77030, USA.
J Bioenerg Biomembr. 2008 Oct;40(5):541-9. doi: 10.1007/s10863-008-9182-6. Epub 2008 Oct 1.
A mechanism for proton pumping is described that is based on chemiosmotic principles and the detailed molecular structures now available for cytochrome oxidases. The importance of conserved water positions and a step-wise gated process of proton translocation is emphasized, where discrete electron transfer events are coupled to proton uptake and expulsion. The trajectory of each pumped proton is the same for all four substrate electrons. An essential role for the His-Tyr cross-linked species is discussed, in gating of the D- and K-channels and as an acceptor/donor of electrons and protons at the binuclear center.
描述了一种基于化学渗透原理和目前已有的细胞色素氧化酶详细分子结构的质子泵浦机制。强调了保守水位置和质子转运的逐步门控过程的重要性，其中离散的电子转移事件与质子的摄取和排出相耦合。对于所有四个底物电子，每个泵浦质子的轨迹都是相同的。讨论了His-Tyr交联物种在D通道和K通道的门控以及作为双核中心电子和质子的受体/供体方面的重要作用。
