Wikström Mårten, Verkhovsky Michael I
Helsinki Bioenergetics Group, Structural Biology and Biophysics Programme, Institute of Biotechnology, University of Helsinki, PB 65 (Viikinkaari 1), FI-00014 University of Helsinki, Finland.
Biochim Biophys Acta. 2007 Oct;1767(10):1200-14. doi: 10.1016/j.bbabio.2007.06.008. Epub 2007 Jul 6.
Recent time-resolved optical and electrometric experiments have provided a sequence of events for the proton-translocating mechanism of cytochrome c oxidase. These data also set limits for the mechanistic, kinetic, and thermodynamic parameters of the proton pump, which are analysed here in some detail. The analysis yields limit values for the pK of the "pump site", its modulation during the proton-pumping process, and suggests its identity in the structure. Special emphasis is made on side-reactions that may short-circuit the pump, and the means by which these may be avoided. We will also discuss the most prominent proton pumping mechanisms proposed to date in relation to these data.
最近的时间分辨光学和电测量实验为细胞色素c氧化酶的质子转运机制提供了一系列事件。这些数据也为质子泵的机制、动力学和热力学参数设定了限制，本文将对其进行详细分析。分析得出了“泵位点”的pK极限值、其在质子泵浦过程中的调节情况，并暗示了其在结构中的身份。特别强调了可能使泵短路的副反应，以及避免这些副反应的方法。我们还将讨论迄今为止提出的与这些数据相关的最突出的质子泵浦机制。
