[亮菌多糖IPS-B2对小鼠腹腔巨噬细胞活性及相关基因转录的影响]

[Effects of Armillariella tabescens polysaccharide IPS-B2 on activity of mouse peritoneal macrophages and transcription of related gene].

作者信息

Luo Xia, Yu Meng-Yao, Xu Xiao-Yan, Zeng Jin, Jiang Nan, Zheng Lin-Yong

机构信息

Cellular and Molecular Lab, Sichuan Institute of Chinese Material Medica, Chengdu 610041, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2008 Jun;33(11):1305-8.

PMID:18831214

Abstract

OBJECTIVE

To observe the effect of IPS-B2 on mouse peritoneal macrophages and the transcription of IL-1beta, IL-6, TNF-alpha and iNOS.

METHOD

ELISA method and Griess method were used to detect the effect of mouse peritoneal macrophages produce cytokines IL-1beta, IL-6, TNF-alpha and cytotoxic effectors NO. The transcription of IL-1beta, IL-6, TNF-alpha and iNOS was detected by real-time RT-PCR method.

RESULT

IPS-B2 could not promote mouse peritoneal macrophage production, but it could significantly improve the IL-1beta, IL-6, TNF-alpha content in mouse peritoneal macrophages culture supernatant, and increase the gene expression of IL-1beta, IL-6, TNF-alpha and iNOS.

CONCLUSION

IPS-B2 can enhance the ability of peritoneal macrophages to excrete bioactive substances and promote the transcription of bioactive substances to antitumor.

摘要

目的

观察IPS - B2对小鼠腹腔巨噬细胞以及白细胞介素 - 1β（IL - 1β）、白细胞介素 - 6（IL - 6）、肿瘤坏死因子 - α（TNF - α）和诱导型一氧化氮合酶（iNOS）转录的影响。

方法

采用酶联免疫吸附测定（ELISA）法和格里斯（Griess）法检测小鼠腹腔巨噬细胞产生细胞因子IL - 1β、IL - 6、TNF - α的效应以及细胞毒性效应分子一氧化氮（NO）。采用实时逆转录 - 聚合酶链反应（real - time RT - PCR）法检测IL - 1β、IL - 6、TNF - α和iNOS的转录情况。