Luo Xia, Xu Xiaoyan, Yu Mengyao, Yang Zhirong, Zheng Linyong
College of Life Sciences, Sichuan University, 610064 Sichuan, China; Sichuan Institute of Chinese Material Medica, Chengdu, 610041 Sichuan, China.
Sichuan Institute of Chinese Material Medica, Chengdu, 610041 Sichuan, China.
Food Chem. 2008 Nov 15;111(2):357-63. doi: 10.1016/j.foodchem.2008.03.076. Epub 2008 Apr 4.
IPS-B2, an intracellular polysaccharide with anti-tumor effect, was isolated from cultured Armillariella tabescens mycelia by hot water extraction, anion-exchange and gel chromatography. Based on the results of paper chromatography (PC), gas chromatography (GC), infra-red (IR) spectroscopy and (13)C NMR, IPS-B2 was characterized as an α-(1→6)-d-glucan with a molecular weight of 49.5kDa. The effects of IPS-B2 on murine peritoneal macrophages were further investigated. The results demonstrated that IPS-B2 induced nitric oxide (NO) and cytokines (TNF-α, IL-1β and IL-6) production in macrophages. The outcomes of real-time reverse transcription-polymerase chain reaction (RT-PCR) proved that the transcribing level of inducible NO synthase (iNOS), TNF-α, IL-1β and IL-6 mRNA in the peritoneal macrophages have been augmented by IPS-B2. These data suggest that the anti-tumor activity of the polysaccharide from A. tabescens may due to activation of macrophage.
IPS - B2是一种具有抗肿瘤作用的细胞内多糖，通过热水提取、阴离子交换和凝胶色谱从培养的亮菌菌丝体中分离得到。基于纸色谱（PC）、气相色谱（GC）、红外（IR）光谱和（13）C核磁共振的结果，IPS - B2被鉴定为分子量为49.5 kDa的α - （1→6）-d - 葡聚糖。进一步研究了IPS - B2对小鼠腹腔巨噬细胞的影响。结果表明，IPS - B2可诱导巨噬细胞产生一氧化氮（NO）和细胞因子（TNF - α、IL - 1β和IL - 6）。实时逆转录 - 聚合酶链反应（RT - PCR）结果证明，IPS - B2增强了腹腔巨噬细胞中诱导型一氧化氮合酶（iNOS）、TNF - α、IL - 1β和IL - 6 mRNA的转录水平。这些数据表明，亮菌多糖的抗肿瘤活性可能归因于巨噬细胞的激活。
