Taupp Daniela E, Nimtz Manfred, Berger Ralf G, Zorn Holger
Zentrum Angewandte Chemie, Institut für Lebensmittelchemie der Leibniz Universitiit Hannover, Wunstorfer Strasse 14, D-30453 Hannover, Germany.
Mycologia. 2008 Jul-Aug;100(4):529-38. doi: 10.3852/07-179r.
Exposition to UV-A light stimulated the growth and synthesis of raspberry ketone in submerged cultures of the basidiomycete Nidula niveotomentosa. To investigate the fungus' response to UV-A light differentially expressed proteins were identified by means of 2D-electrophoresis. Light induced proteins were de novo sequenced by ESI-MS/MS spectrometry, and the encoding nucleotide sequences were cloned from cDNA or genomic DNA. The spectrum of UV-A light-induced proteins comprised several stress-related proteins including a catalase, heat-shock proteins, glutathione S-transferases and proteasomes. In addition, growth-related enzymes of the citric cycle were found to be up-regulated as a response to irradiation with UV-A.
