Ando A, Harada A, Miura K, Tamai Y
Laboratory of Forest Resource Biology, Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo 060-8589, Japan.
Curr Genet. 2001 May;39(3):190-7. doi: 10.1007/s002940100193.
A cDNA clone of the gene fvh1 was previously isolated from a preprimordial cDNA library made from the basidiomycete Flammulina velutipes. Sequence analysis showed that fvh1 encoded for a hydrophobin, a small fungal protein usually secreted by filamentous fungi. FVH1 had a highly conserved arrangement of eight cysteine residues, a putative N-terminal signal sequence and a hydropathy pattern characteristics of class I hydrophobin. A genomic fvh1 clone was isolated from a F. velutipes genomic DNA library and sequenced. Several putative promoter elements and three small introns of fvh1 were identified in this clone. Southern blot analysis of genomic DNA showed that fvh1 was a single copy gene. Northern analysis indicated that fvh1 was specifically and abundantly expressed in mycelia after the induction of fruiting and during fruit body initiation. It was not expressed in mycelia before the induction of fruiting or in mature fruit bodies.
先前从担子菌金针菇的原始前cDNA文库中分离出了基因fvh1的一个cDNA克隆。序列分析表明，fvh1编码一种疏水蛋白，这是一种通常由丝状真菌分泌的小型真菌蛋白。FVH1具有八个半胱氨酸残基的高度保守排列、一个推定的N端信号序列以及I类疏水蛋白的亲水性模式特征。从金针菇基因组DNA文库中分离出一个基因组fvh1克隆并进行了测序。在该克隆中鉴定出了几个推定的启动子元件和fvh1的三个小内含子。基因组DNA的Southern印迹分析表明，fvh1是一个单拷贝基因。Northern分析表明，fvh1在子实体诱导后和子实体起始期间在菌丝体中特异性且大量表达。在子实体诱导前的菌丝体或成熟子实体中不表达。
