Yang Chi, Ma Lu, Ying Zhenghe, Jiang Xiaoling, Lin Yanquan
Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, 350014, China.
National and Local Joint Engineering Research Center for Breeding & Cultivation of Featured Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, 350014, China.
Curr Microbiol. 2017 Apr;74(4):469-475. doi: 10.1007/s00284-017-1218-x. Epub 2017 Feb 22.
Light is a necessary environmental factor for fruit body formation and development of the cauliflower mushroom Sparassis latifolia, a well-known edible and medicinal fungus. In this study, we firstly characterized the SP-C strain, which belonged to S. latifolia. And then we cloned and sequenced a photoreceptor gene (Slwc-1) from S. latifolia. The product of Slwc-1, SlWC-1 (872 aa residues) contained a coiled-coil region, a LOV domain, and two PAS domains. Phylogenetic tree result showed that SLWC-1 was most close to GfWC-1 from Grifola frondosa in edible and medicinal fungus. The Slwc-1 gene was found to be enhanced by light. This report will help to open the still-unexplored field of fruit body development for this fungus.
光是著名的食药用真菌阔叶白耙齿菌子实体形成和发育所必需的环境因素。在本研究中，我们首先对属于阔叶白耙齿菌的SP-C菌株进行了特性分析。然后我们从阔叶白耙齿菌中克隆并测序了一个光受体基因（Slwc-1）。Slwc-1的产物SlWC-1（872个氨基酸残基）包含一个卷曲螺旋区域、一个LOV结构域和两个PAS结构域。系统发育树结果表明，在食药用真菌中，SLWC-1与灰树花的GfWC-1最为接近。发现Slwc-1基因受光增强。本报告将有助于开启对这种真菌子实体发育尚未探索的领域。
