Sahin Figen Kir, Cosar Emine, Koken Gulengul, Toy Hatice, Basarali Kemal, Buyukbas Sadik
Department of Obstetrics and Gynecology, Faculty of Medicine, Afyonkarahisar Kocatepe University, Afyonkarahisar, Turkey.
J Obstet Gynaecol Res. 2008 Oct;34(5):794-800. doi: 10.1111/j.1447-0756.2008.00846.x.
We investigated the effects of aprotinin on reperfusion injury in a controlled experimental rat torsion-detorsion model.
Thirty-two female Sprague-Dawley rats were divided into four groups. Sham operation was performed in group I; in group II only ovarian torsion was performed. In group III, torsion-detorsion was performed, plus 3 mL/kg saline was injected i.v. 30 min before detorsion. In group IV, torsion-detorsion was performed, plus aprotinin (30,000 KIU/kg) was injected 30 min before detorsion. Rats in the torsion group were killed after 360 degrees clockwise adnexial torsion for 3 h, and the ovaries were harvested. After 3 h of adnexial detorsion, the rats in the saline and aprotinin groups were killed and the adnexa were surgically removed.
Ovarian tissue damage scores were significantly different among groups. Ovarian tissue and serum malondialdehyde levels in group III were significantly higher than those of groups I and IV (P<0.05). The serum levels of superoxide dismutase in group III were significantly lower than those of groups I and IV (P=0.01). Tissue and serum xanthine oxidase, nitric oxide, and tissue superoxide dismutase levels were comparable among groups (P>0.05).
Aprotinin attenuates ischemia-reperfusion injury in a rat adnexial torsion-detorsion model.
在可控的实验性大鼠卵巢扭转-复位模型中，研究抑肽酶对再灌注损伤的影响。
将32只雌性Sprague-Dawley大鼠分为四组。第一组进行假手术；第二组仅进行卵巢扭转。第三组进行扭转-复位，在复位前30分钟静脉注射3 mL/kg生理盐水。第四组进行扭转-复位，在复位前30分钟静脉注射抑肽酶（30,000 KIU/kg）。扭转组大鼠在顺时针附件扭转360度3小时后处死，摘取卵巢。附件复位3小时后，处死生理盐水组和抑肽酶组大鼠，并手术切除附件。
各组卵巢组织损伤评分有显著差异。第三组卵巢组织和血清丙二醛水平显著高于第一组和第四组（P<0.05）。第三组血清超氧化物歧化酶水平显著低于第一组和第四组（P=0.01）。各组组织和血清黄嘌呤氧化酶、一氧化氮以及组织超氧化物歧化酶水平相当（P>0.05）。
在大鼠附件扭转-复位模型中，抑肽酶可减轻缺血-再灌注损伤。
