Hascalik Seyma, Celik Onder, Turkoz Yusuf, Hascalik Mehmet, Cigremis Yilmaz, Mizrak Bulent, Yologlu Saim
Department of Obstetrics and Gynecology, School of Medicine, Malatya, Turkey.
Gynecol Obstet Invest. 2004;57(4):218-23. doi: 10.1159/000076760. Epub 2004 Feb 11.
The aim of this study is to investigate the effects of resveratrol on histopathological changes, antioxidant status and lipid peroxidation, in torsion-detorsion injury in rat ovaries.
To determine whether ischemia followed by reperfusion can induce ovarian oxidative damage, we created a model of adnexal ischemia-reperfusion by using rats. Ischemia was induced by unilateral occlusion of the tubo-ovarian vessels for 3 h. Reperfusion was achieved by releasing the occlusion and restoring the circulation for 3 h. Thirty-two adult female albino rats were divided equally into 4 groups: sham operation, torsion, saline/detorsion and resveratrol/detorsion. Rats in the torsion group were killed after 360 degrees clockwise adnexal torsion for 3 h. Resveratrol was injected intraperitoneally 30 min before detorsion in the resveratrol/detorsion group, and saline was administered in the saline/detorsion group. After 3 h of adnexal detorsion in both of these groups, the rats were killed and adnexa were removed. The tissue levels of malondialdehyde, reduced glutathione and xanthine oxidase activity were measured.
Malondialdehyde and xanthine oxidase levels in the saline/detorsion group were increased significantly when compared to the torsion and sham operation groups (p < 0.001). Malondialdehyde levels in the resveratrol group were lower than in the saline/detorsion group, and differences between the two groups were statistically significant (p < 0.001). Xanthine oxidase levels in the resveratrol group were lower than in the saline/detorsion and torsion groups, and differences between these groups were statistically significant (p < 0.001). Reduced glutathione levels in the saline/detorsion group were decreased significantly when compared to the torsion and sham operation groups. Reduced glutathione levels in the resveratrol group were significantly higher than in the saline/detorsion group (p < 0.006). Histological examination showed a significant improvement in ovarian morphology in the resveratrol-treated rats compared with the ischemia and ischemia-reperfusion groups.
Our results demonstrated that intraperitoneal resveratrol administration reduced the lipid peroxidation products of ischemic rats and ovarian damage was reduced as indicated by histological examination.
本研究旨在探讨白藜芦醇对大鼠卵巢扭转-复位损伤的组织病理学变化、抗氧化状态及脂质过氧化的影响。
为确定缺血再灌注是否会诱导卵巢氧化损伤，我们使用大鼠建立了附件缺血-再灌注模型。通过单侧阻断输卵管-卵巢血管3小时诱导缺血。松开阻断并恢复循环3小时实现再灌注。32只成年雌性白化大鼠平均分为4组：假手术组、扭转组、生理盐水/复位组和白藜芦醇/复位组。扭转组大鼠在顺时针附件扭转360度3小时后处死。白藜芦醇/复位组在复位前30分钟腹腔注射白藜芦醇，生理盐水/复位组注射生理盐水。在这两组进行附件复位3小时后，处死大鼠并取出附件。测量丙二醛、还原型谷胱甘肽的组织水平及黄嘌呤氧化酶活性。
与扭转组和假手术组相比，生理盐水/复位组的丙二醛和黄嘌呤氧化酶水平显著升高（p < 0.001）。白藜芦醇组的丙二醛水平低于生理盐水/复位组，两组间差异具有统计学意义（p < 0.001）。白藜芦醇组的黄嘌呤氧化酶水平低于生理盐水/复位组和扭转组，这些组间差异具有统计学意义（p < 0.001）。与扭转组和假手术组相比，生理盐水/复位组的还原型谷胱甘肽水平显著降低。白藜芦醇组的还原型谷胱甘肽水平显著高于生理盐水/复位组（p < 0.006）。组织学检查显示，与缺血组和缺血-再灌注组相比，白藜芦醇治疗的大鼠卵巢形态有显著改善。
我们的结果表明，腹腔注射白藜芦醇可减少缺血大鼠的脂质过氧化产物，组织学检查表明卵巢损伤减轻。
