用于评估麻疹疫苗免疫原性的蚀斑减少中和试验（PRNT）与麻疹病毒特异性IgG酶联免疫吸附测定（ELISA）的比较
Comparison of plaque reduction neutralisation test (PRNT) and measles virus-specific IgG ELISA for assessing immunogenicity of measles vaccination.
作者信息
Cohen B J, Doblas D, Andrews N
机构信息
Virus Reference Department, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, United Kingdom.
出版信息
Vaccine. 2008 Nov 25;26(50):6392-7. doi: 10.1016/j.vaccine.2008.08.074. Epub 2008 Oct 1.
BACKGROUND
Plaque reduction neutralisation test (PRNT) is a gold standard assay for measles antibodies. PRNT, however, is time-consuming and more difficult to standardise than ELISA.
METHODS
PRNT results were obtained on pre- and post-vaccination sera from 501 measles-vaccinated infants. From this cohort a sub-set of 210 infants were selected for testing by ELISA, which was performed either manually or by an automated processor. Results were corrected for sampling proportion before comparing the assays.
RESULTS
Seroconversion was detected by PRNT in 454 infants of whom 324 were estimated to have seroconverted by manual ELISA and 191 by automated ELISA It is recommended that ELISA negative post-vaccination samples are re-tested by PRNT to detect all seroconversions.
背景
蚀斑减少中和试验（PRNT）是检测麻疹抗体的金标准检测方法。然而，PRNT耗时且比酶联免疫吸附测定（ELISA）更难标准化。
方法
获得了501名接种麻疹疫苗婴儿接种疫苗前后血清的PRNT结果。从该队列中选取210名婴儿的子集通过ELISA进行检测，ELISA检测采用手动或自动分析仪进行。在比较检测方法之前，对结果进行抽样比例校正。
结果
通过PRNT检测到454名婴儿发生血清转化，其中估计324名婴儿通过手动ELISA发生血清转化，191名婴儿通过自动ELISA发生血清转化。建议对疫苗接种后ELISA检测为阴性的样本通过PRNT重新检测，以检测所有血清转化情况。
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