麻疹免疫检测：两种麻疹IgG酶联免疫吸附测定法与蚀斑减少中和试验的比较
Measles immunity testing: comparison of two measles IgG ELISAs with plaque reduction neutralisation assay.
作者信息
Cohen B J, Parry R P, Doblas D, Samuel D, Warrener L, Andrews N, Brown D
机构信息
Virus Reference Department, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5HT, UK.
出版信息
J Virol Methods. 2006 Feb;131(2):209-12. doi: 10.1016/j.jviromet.2005.08.001. Epub 2005 Sep 26.
Two commercial IgG ELISAs, one based on recombinant nucleocapsid antigen and one based on cell culture grown native virus antigens, were evaluated for measles immunity testing by comparison with plaque reduction neutralisation test (PRNT). Qualitative results of the two ELISAs showed 92% agreement with those of PRNT. The sensitivity of the two ELISAs was 89.6%. False negative ELISA results were obtained in 10% of sera, mainly sera containing low levels of neutralising antibody. The specificity of both ELISAs was 100%. Measles IgG ELISAs perform adequately for immunity testing, correctly identifying seronegative individuals for vaccination.
通过与蚀斑减少中和试验（PRNT）比较，对两种商用IgG酶联免疫吸附测定（ELISA）进行了评估，一种基于重组核衣壳抗原，另一种基于细胞培养产生的天然病毒抗原，用于麻疹免疫检测。两种ELISA的定性结果与PRNT的结果一致性为92%。两种ELISA的敏感性为89.6%。10%的血清出现ELISA假阴性结果，主要是含有低水平中和抗体的血清。两种ELISA的特异性均为100%。麻疹IgG ELISA在免疫检测中表现良好，能够正确识别血清阴性个体以进行疫苗接种。
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