酶联免疫法检测麻疹IgG抗体试剂盒与蚀斑减少中和试验的比较与评价
[Comparison and evaluation of enzyme-linked immunization assay kits with plague reduction neutralization test for detection of measles IgG antibody].
作者信息
Mao Nai-ying, Zhu Zhen, Jiang Xiao-hong
机构信息
World Health Organization Western Pacific Region Regional Reference Measles Laboratory State Key Laboratory for Molecular Virology & Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
出版信息
Zhongguo Yi Miao He Mian Yi. 2009 Jun;15(3):215-8.
OBJECTIVE
To evaluate and comparison of 2 commercial ELISA kits (Vrion/Serion kit and IBL kit) which ELISA kit will be used in sero-epidemiological survey in China in 2006 by Plaque Reduction Neutralization Test (PRNT).
METHODS
52 serum panel which contain different levels of measles antibody were used.
RESULTS
The results showed that the sensitivity, PPV and accurate of Virion/Serion kit achieved highest score when taking PRNT as "gold standard". Virion/Serion kit showed good relationship with PRNT titer: the correlation coefficients are 0.878 (P<0.01). IBL kit get lower correlation coefficients 0.850 (P<0.01). The test value and mean of unit of serum antibody increased along with neutralizing titers increased.
CONCLUSION
The Virion/Serion kit is good in sensitivity and specificity. It has quantitative for single well. The kit is suitable for sera epidemiology survey to detection of automatic workstation in China.
目的
通过蚀斑减少中和试验（PRNT）评估并比较两种商用ELISA试剂盒（Vrion/Serion试剂盒和IBL试剂盒），以确定哪种试剂盒将用于2006年中国的血清流行病学调查。
方法
使用了52份含有不同水平麻疹抗体的血清样本。
结果
结果显示，以PRNT为“金标准”时，Vrion/Serion试剂盒的敏感性、阳性预测值和准确性得分最高。Vrion/Serion试剂盒与PRNT滴度显示出良好的相关性：相关系数为0.878（P<0.01）。IBL试剂盒的相关系数较低，为0.850（P<0.01）。血清抗体单位的检测值和平均值随着中和滴度的增加而增加。
结论
Vrion/Serion试剂盒在敏感性和特异性方面表现良好。它对单孔有定量功能。该试剂盒适用于中国血清流行病学调查中自动工作站的检测。
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