异常汉逊酵母中抗霉素A诱导型mRNA的cDNA分子克隆及其在抗氰呼吸中的作用

Molecular cloning of cDNA for antimycin A-inducible mRNA and its role in cyanide-resistant respiration in Hansenula anomala.

作者信息

Sakajo S, Minagawa N, Komiyama T, Yoshimoto A

机构信息

Department of Biochemistry, Niigata College of Pharmacy, Japan.

出版信息

Biochim Biophys Acta. 1991 Aug 27;1090(1):102-8. doi: 10.1016/0167-4781(91)90043-l.

DOI:10.1016/0167-4781(91)90043-l

PMID:1883836

Abstract

A cDNA for mRNA induced by antimycin A in Hansenula anomala was cloned. The mRNA for the cDNA was expressed in the yeast under the conditions expressing the cyanide-resistant respiration activity. The nucleotide sequence revealed a long open reading frame of 342 codons encoding a protein with a molecular weight of 40,282 in the cDNA. An antibody recognizing the protein encoded by the open reading frame was produced. Immunoblotting of H. anomala proteins with this antibody showed that a 36 kDa protein localized in mitochondria was a mature form of the protein encoded by the cDNA. It is suggested that the cloned cDNA encodes a protein involved in the cyanide-resistant respiratory pathway.

摘要

克隆了异常汉逊酵母中抗霉素A诱导产生的mRNA的cDNA。该cDNA的mRNA在表达抗氰呼吸活性的条件下于酵母中表达。核苷酸序列显示该cDNA中有一个长342个密码子的开放阅读框，编码一个分子量为40282的蛋白质。制备了一种识别由该开放阅读框编码的蛋白质的抗体。用此抗体对异常汉逊酵母蛋白质进行免疫印迹分析表明，定位于线粒体的一种36 kDa蛋白质是该cDNA编码蛋白质的成熟形式。提示克隆的cDNA编码一种参与抗氰呼吸途径的蛋白质。