Sakajo S, Minagawa N, Yoshimoto A
Department of Biochemistry, Niigata College of Pharmacy, Japan.
FEBS Lett. 1993 Mar 8;318(3):310-2. doi: 10.1016/0014-5793(93)80535-3.
The cyanide-resistant respiratory pathway is induced by respiratory inhibitors in the yeast Hansenula anomala. A monoclonal antibody against the alternative oxidase in the higher plant Sauromatum guttatum cross-reacted with a 36-kDa mitochondrial protein induced by antimycin A in H. anomala and with a protein encoded by a cDNA which was previously cloned for an antimycin A-inducible mRNA in the yeast. There was a similarity in the amino acid sequence between the cDNA-encoded protein and the plant alternative oxidase protein. We propose that the 36-kDa mitochondrial protein encoded by the cDNA is a component of alternative oxidase in H. anomala.
抗氰呼吸途径在异常汉逊酵母中由呼吸抑制剂诱导产生。一种针对高等植物斑龙芋中交替氧化酶的单克隆抗体，与抗霉素A在异常汉逊酵母中诱导产生的一种36 kDa线粒体蛋白发生交叉反应，也与一个cDNA编码的蛋白发生交叉反应，该cDNA先前是为酵母中抗霉素A诱导型mRNA而克隆的。cDNA编码的蛋白与植物交替氧化酶蛋白的氨基酸序列存在相似性。我们推测，cDNA编码的36 kDa线粒体蛋白是异常汉逊酵母中交替氧化酶的一个组分。
