Im Sung Gap, Bong Ki Wan, Kim Byeong-Su, Baxamusa Salmaan H, Hammond Paula T, Doyle Patrick S, Gleason Karen K
Department of Chemical Engineering and Institute for Soldiers Nanotechnology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
J Am Chem Soc. 2008 Nov 5;130(44):14424-5. doi: 10.1021/ja806030z. Epub 2008 Oct 8.
simple method to fabricate a multifunctional patterned platform on the nanometer scale is demonstrated. The platform contains two reactive functional groups on the surface: one is an acetylene group which can be functionalized via click chemistry, and the other is an amine group which can also be functionalized by classic carbodiimide chemistry with N-hydroxysuccinimide (NHS). The click-active and amine surface could be obtained from polymer coating of poly(propargyl methacrylate) (PPMA) via initiated chemical vapor deposition (iCVD) and poly(allylamine) (PAAm) via a plasma polymerization process, respectively, utilizing commercially available monomers. A capillary force lithography (CFL) process was applied on a stacked film of a PPMA layer on PAAm, and CFL could selectively pattern PPMA maintaining the bottom PAAm layer intact, which completes the multifunctional nanopatterns. The minimum feature size of this nanopattern was 110 nm. The entire fabrication process is solventless and low temperature, which can minimize the loss of functionalities. The click and NHS reactions are highly orthogonal to each other so that nonspecific immobilization can be minimized. These advantageous characteristics enable the covalent functionalization of two independent components in a one-pot functionalization process in self-recognized way. The one-pot orthogonal functionalization was performed in an aqueous solution at room temperature, which is biocompatible. Considering the versatility and generality of the reactions used here, we believe this platform can be easily extended to various biodevice applications.
展示了一种在纳米尺度上制造多功能图案化平台的简单方法。该平台表面含有两种反应性功能基团：一种是乙炔基，可通过点击化学进行功能化；另一种是胺基，也可通过与N-羟基琥珀酰亚胺（NHS）的经典碳二亚胺化学进行功能化。具有点击活性的表面和胺基表面可分别通过引发化学气相沉积（iCVD）由聚（甲基丙烯酸炔丙酯）（PPMA）的聚合物涂层以及通过等离子体聚合过程由聚（烯丙胺）（PAAm）获得，使用的是市售单体。在PAAm上的PPMA层的堆叠膜上应用了毛细力光刻（CFL）工艺，并且CFL可以选择性地对PPMA进行图案化，同时保持底部的PAAm层完整，从而完成多功能纳米图案。这种纳米图案的最小特征尺寸为110nm。整个制造过程是无溶剂且低温的，这可以使功能损失最小化。点击反应和NHS反应彼此高度正交，从而可以将非特异性固定最小化。这些有利特性使得能够在一锅功能化过程中以自识别方式对两个独立组分进行共价功能化。一锅正交功能化在室温下的水溶液中进行，该水溶液具有生物相容性。考虑到这里使用的反应的通用性和普遍性，我们相信这个平台可以很容易地扩展到各种生物器件应用。
