Webster D, Haase D, Marrie T J, Campbell N, Pettipas J, Davidson R, Hatchette T F
Division of Infectious Diseases, Department of Medicine, Saint John Regional Hospital, Saint John, New Brunswick, Canada.
Epidemiol Infect. 2009 May;137(5):744-51. doi: 10.1017/S0950268808001404. Epub 2008 Oct 8.
In Atlantic Canada, the traditional risk factor for acquisition of Q fever infection has been exposure to infected parturient cats or newborn kittens. In this study we describe the first case of Q fever in Nova Scotia acquired as a result of direct exposure to sheep. A serosurvey of the associated flock was undertaken using an indirect immunofluorescence assay (IFA) testing for antibodies to phase I and phase II Coxiella burnetii antigens. This serosurvey revealed that 23 of 46 sheep (50%) were seropositive for the phase II antibody. Four of these sheep had titres of 1:64 including three nursing ewes, one of which had delivered two lambs that died shortly after delivery. Only one ewe had phase I antibodies but had the study's highest phase II antibody titre (1:128). Molecular studies using polymerase chain reaction (PCR) failed to detect C. burnetii DNA in any of the milk specimens.
在加拿大大西洋地区，感染Q热的传统风险因素是接触受感染的母猫或新生小猫。在本研究中，我们描述了新斯科舍省首例因直接接触绵羊而感染Q热的病例。使用间接免疫荧光法（IFA）对相关羊群进行血清学调查，检测针对I相和II相伯氏考克斯体抗原的抗体。该血清学调查显示，46只绵羊中有23只（50%）II相抗体血清呈阳性。其中4只绵羊的滴度为1:64，包括3只哺乳母羊，其中1只母羊产下2只羊羔，羊羔在出生后不久死亡。只有1只母羊有I相抗体，但具有该研究中最高的II相抗体滴度（1:128）。使用聚合酶链反应（PCR）的分子研究未能在任何牛奶样本中检测到伯氏考克斯体DNA。
