Pelclová Daniela, Fenclová Zdenka, Kacer Petr, Kuzma Marek, Navrátil Tomás, Lebedová Jindriska
1st Medical Faculty, Department of Occupational Medicine, Charles University of Prague, Prague, Czech Republic.
Ind Health. 2008 Oct;46(5):484-9. doi: 10.2486/indhealth.46.484.
Asbestosis and pleural plaques exhibit unpredictable but progressive development, and there are no markers routinely available to measure their prognosis. Asbestos exposure induces the generation of reactive oxygen species, and 8-isoprostane is involved in experimental asbestos-related lung toxicity. This oxidative stress marker was measured in exhaled breath condensate (EBC) in 92 former asbestos workers with mean age 68.8+/-1.7 yr and mean duration of asbestos exposure 24.1+/-2.0 yr. The control group had 46 subjects with mean age 65.2+/-3.3 yr. The mean level of 8-isoprostane, analyzed by liquid chromatography-electrospray ionization-mass spectrometry, was higher in asbestos-exposed subjects (69.5+/-6.6 pg/ml, p=0.0001) compared with the control group, where the concentration was 47.0+/-7.8 pg/ml. The results presented support the hypothesis that oxidative stress due to asbestos is the main cause of increased 8-isoprostane in EBC. Measurement of 8-isoprostane in EBC is a promising non-invasive means for assessing the activity of asbestos-induced diseases.
石棉沉着病和胸膜斑呈现出不可预测但进行性的发展，并且没有常规可用的标志物来衡量其预后。石棉暴露会诱导活性氧的产生，8-异前列腺素参与了与石棉相关的实验性肺毒性。在92名平均年龄为68.8±1.7岁且平均石棉暴露时长为24.1±2.0年的前石棉工人的呼出气冷凝物（EBC）中测量了这种氧化应激标志物。对照组有46名平均年龄为65.2±3.3岁的受试者。通过液相色谱-电喷雾电离-质谱分析，石棉暴露受试者的8-异前列腺素平均水平（69.5±6.6 pg/ml，p = 0.0001）高于对照组，对照组的浓度为47.0±7.8 pg/ml。所呈现的结果支持了这样的假设，即石棉引起的氧化应激是EBC中8-异前列腺素增加的主要原因。在EBC中测量8-异前列腺素是评估石棉诱导疾病活性的一种有前景的非侵入性方法。
