Tiryaki B S, Tasliyurt T, Yelken B M, Sahin S, Kutluturk F, Koseoglu H I, Ozturk B, Yilmaz A, Sahin S
Department of Internal Medicine, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
Niger J Clin Pract. 2014 May-Jun;17(3):356-60. doi: 10.4103/1119-3077.130240.
There have been limited numbers of studies on patients with chronic kidney disease (CKD) to determine oxidative stress in exhaled breath condensate (EBC). Those two studies have been carried out on hemodialysis patients, and hydrogen peroxide and nitric oxide have been studied in order to show oxidative stress on EBC.
We investigated oxidative stress in EBC evaluating 8-isoprostane levels on different stages of CKD.
A total of 81 patients with 2-4 CKD stages have been evaluated prospectively. The patients have been categorized into three groups according to their CKD stages. For biochemical analysis, blood and breathing air samples were taken. 8-isoprostane has been measured using immunoassay method as the indicator of oxidative stress in EBC.
8-isoprostane values were 8.19 ± 4.56, 13.89 ± 8.70, and 14.20 ± 10.68 pg/min group 1, 2, and 3, respectively; and the EBC 8-isoprostane levels increased significantly as CKD stages advanced (P0 = 0.018). There was a statistically significant reverse correlation between 8-isoprostane and glomerular filtration rate (GFR; r = -0.275; P = 0.014), but not between 8-isoprostane and C-reactive protein (r = -0.183; P = 0.177).
We determined the level of 8-isoprostane in EBC of patients with different stages of CKD and showed that the level of 8-isoprostane significantly increased through the progress of CKD. We consider that our study is important because there have been limited number of studies that evaluate oxidative stress in CKD using EBC which is a noninvasive method.
关于慢性肾脏病（CKD）患者呼出气体冷凝液（EBC）中氧化应激的研究数量有限。这两项研究是针对血液透析患者进行的，并且对过氧化氢和一氧化氮进行了研究，以显示EBC中的氧化应激。
我们通过评估CKD不同阶段的8-异前列腺素水平来研究EBC中的氧化应激。
前瞻性评估了总共81例2-4期CKD患者。根据CKD阶段将患者分为三组。为了进行生化分析，采集了血液和呼吸气体样本。使用免疫测定法测量8-异前列腺素，作为EBC中氧化应激的指标。
第1、2和3组的8-异前列腺素值分别为8.19±4.56、13.89±8.70和14.20±10.68 pg/分钟；并且随着CKD阶段的进展，EBC中8-异前列腺素水平显著升高（P0 = 0.018）。8-异前列腺素与肾小球滤过率（GFR；r = -0.275；P = 0.014）之间存在统计学上显著的负相关，但8-异前列腺素与C反应蛋白之间不存在负相关（r = -0.183；P = 0.177）。
我们测定了不同阶段CKD患者EBC中8-异前列腺素的水平，并表明随着CKD的进展，8-异前列腺素水平显著升高。我们认为我们的研究很重要，因为使用EBC这种非侵入性方法评估CKD中氧化应激的研究数量有限。
