Kim Young Heui, Kim Ki Ho, Han Chang Sung, Park Sun Hee, Yang Hong Chul, Lee Bang Yong, Eom Sang-Yong, Kim Young-Sil, Kim Jong-Heon, Lee Nam Ho
R&D Center, Bioland Ltd., Songjeong, Byongchon, Cheonan-si, Chungnam, 330-863.
J Cosmet Sci. 2008 Sep-Oct;59(5):419-30.
Crinum asiaticum Linne var. japonicum has long been used as a rheumatic remedy, as an anti-pyretic and as an anti-ulcer treatment, and for the alleviation of local pain and fever in Korea and Malaysia. In order to investigate the possibility of Crinum asiaticum Linne var. japonicum extract as a cosmetic ingredient, we measured its anti-inflammatory effect by its inhibition of iNOS (inducible nitric oxide synthase) and the release of PGE2, IL-6, and IL-8. We also measured its anti-allergic effect by its inhibition of beta-hexosamidase release. An HPLC experiment after extraction with 95% EtOH at pH 3.5 showed that Crinum asiaticum Linne var. japonicum was mainly composed of lycorine (up to 1%), a well-known immunosuppressor. The content of lycorine varied, depending on the type of plant tissue analyzed and the extraction method. In an anti-inflammatory assay for inhibition of nitric oxide formation on lipopolysaccharide (LPS)-activated mouse macrophage RAW 264.7 cells, the ethanol extract of Crinum asiaticum showed an inhibitory activity of NO production in a dose-dependent manner (IC50 = 58.5 microg/ml). Additional study by RT-PCR demonstrated that the extract of Crinum asiaticum significantly suppressed the expression of the iNOS gene. Moreover, the extract of Crinum asiaticum did not show any cytotoxicity, but did show a cell proliferation effect against LPS (a 10 approximately 60% increase in cell viability). In an assay to determine inhibition of the H2O2-activated release of PGE2, IL-6, and IL-8 in human normal fibroblast cell lines, the release of PGE2 and IL-6 was almost completely inhibited above concentrations of 0.05% and 1%, respectively. Moreover, the release of IL-8 was completely inhibited over the entire range of concentration (>0.0025%). In order to investigate the skin-sensitizing potentials of the extract of Crinum asiaticum, a human clinical test was performed after repeated epicutaneous 48-h applications under an occlusive patch (RIPT). The repeated and single cutaneous applications of Crinum asiaticum Linne var. japonicum extract under the occlusive patch did not provoke any cumulative irritation and sensitization reactions. The result showed that the extract of Crinum asiaticum Linne var. japonicum has a sufficient anti-inflammatory effect. Therefore, Crinum asiaticum Linne var. japonicum extract may be useful for development as an ingredient in cosmetic products.
文殊兰长期以来被用作治疗风湿病的药物、退烧药和抗溃疡药物，在韩国和马来西亚还用于缓解局部疼痛和发烧。为了研究文殊兰提取物作为化妆品成分的可能性，我们通过抑制诱导型一氧化氮合酶（iNOS）以及前列腺素E2（PGE2）、白细胞介素-6（IL-6）和白细胞介素-8（IL-8）的释放来测定其抗炎作用。我们还通过抑制β-己糖胺酶的释放来测定其抗过敏作用。在pH 3.5条件下用95%乙醇提取后进行的高效液相色谱实验表明，文殊兰主要成分是石蒜碱（含量高达1%），一种著名的免疫抑制剂。石蒜碱的含量因所分析的植物组织类型和提取方法而异。在脂多糖（LPS）激活的小鼠巨噬细胞RAW 264.7细胞上进行的一氧化氮形成抑制抗炎试验中，文殊兰乙醇提取物呈剂量依赖性抑制一氧化氮生成（半数抑制浓度=58.5微克/毫升）。通过逆转录聚合酶链反应（RT-PCR）进行的进一步研究表明，文殊兰提取物显著抑制iNOS基因的表达。此外，文殊兰提取物未显示任何细胞毒性，但对LPS表现出细胞增殖作用（细胞活力增加10%至60%）。在测定对人正常成纤维细胞系中过氧化氢激活的PGE2、IL-6和IL-8释放的抑制试验中，分别在浓度高于0.05%和1%时，PGE2和IL-6的释放几乎完全被抑制。此外，在整个浓度范围内（>0.0025%），IL-8的释放被完全抑制。为了研究文殊兰提取物的皮肤致敏潜力，在封闭贴片（重复开放涂抹试验，RIPT）下进行48小时重复经皮涂抹后进行了人体临床试验。在封闭贴片下重复和单次皮肤涂抹文殊兰提取物均未引发任何累积刺激和致敏反应。结果表明，文殊兰提取物具有足够强大的抗炎作用。因此，文殊兰提取物可能有助于开发成为化妆品中的一种成分。
