Il'duganov N A, Chesnokov V N, Mertvetsov N P, Blinova N N
Vopr Med Khim. 1977 Mar-Apr;23(2):226-32.
Two isoenzymes of tyrosine aminotransferase (induced and noninduced by hydrocortisone were isolated from rat liver tissue and subjected to chromatographic purification. Specific antibodies with high titer were obtained after immunization of rabbits by the inducible isoenzyme. The antigenic properties of the obtained tyrosine aminotransferase isoenzymes were compared after double immunodiffusion in agar and immunoelectrophoresis. The purified induced and non-induced isoenzymes were shown to possess both similar and different antigenic determinants. The data obtained suggest that differences in antigenic properties of the tyrosine aminotransferase isoenzymes reflect those variations in their structure, which are expressed as various thermostability, sensitivity to proteolytic enzymes and substrate specificity.
从大鼠肝脏组织中分离出两种酪氨酸转氨酶同工酶（氢化可的松诱导型和非诱导型），并进行色谱纯化。用诱导型同工酶免疫兔子后获得了高滴度的特异性抗体。在琼脂双免疫扩散和免疫电泳后比较了所得酪氨酸转氨酶同工酶的抗原特性。纯化后的诱导型和非诱导型同工酶显示出既有相似的抗原决定簇，也有不同的抗原决定簇。所得数据表明，酪氨酸转氨酶同工酶抗原特性的差异反映了其结构上的那些变化，这些变化表现为不同的热稳定性、对蛋白水解酶的敏感性和底物特异性。
