Chesnokov V N, Blinova N N, Mertvetsov N P
Biokhimiia. 1978;43(4):625-30.
A fraction of rat liver polyribosomes is isolated, which in its immunochemical characteristics considerably enriched with polyribosomes capable to synthesize hydrocortisone-induced liver tyrosine aminotransferase isoenzyme. This specific polyribosome fraction was purified by immunochemical fractionation of total liver polyribosomes using indirect precipitation. The content of polyribosomes in immunoprecipitates comprise 0.4-0.8% of its initial amount (before immunochemical fractionation). The ratio of specific polyribosomes in immunoprecipitates varies from 20 to 45%, which corresponds to 25-100-fold purification. The data obtained suggest that the method of indirect precipitation can be an efficient step in the isolation procedure of individual mRNA.
分离出一部分大鼠肝脏多核糖体，其免疫化学特性表明，该多核糖体富含能够合成氢化可的松诱导的肝脏酪氨酸转氨酶同工酶的多核糖体。通过间接沉淀法对肝脏总多核糖体进行免疫化学分级分离，从而纯化该特定的多核糖体组分。免疫沉淀物中多核糖体的含量占其初始量（免疫化学分级分离前）的0.4 - 0.8%。免疫沉淀物中特定多核糖体的比例在20%至45%之间变化，这相当于25至100倍的纯化。所得数据表明，间接沉淀法可能是分离单个mRNA过程中的一个有效步骤。
